| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 500,096 | 0 | C | T | 53.3% | 1.8 / 18.7 | 15 | K19K (AAG→AAA) | fsr | fosmidomycin MFS transporter |
| Reads supporting (aligned to +/- strand): ref base C (6/1); new base T (0/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAA > NZ_CP009273/499960‑500218 | tCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCt < 2:216792/138‑1 (MQ=255) cATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACg > 2:492961/1‑137 (MQ=255) tCAGAAAAATTTCTGACTGAAGCAGCGGATAAACCGCAAAAATCAGCGATTGGATCATGTCTTTCAGCAGATGTGAGAGGCTGATACCACCTAAAATCCCAAACGATGTGCGGGCTTTGGTCGTTGACGCGGCCGcgcc < 1:330889/139‑1 (MQ=255) agagAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGcgcccg > 1:324324/1‑139 (MQ=255) aaaatTTCTGCCTGAAGCAGCGGATAAGCCGCAAGAACCAGCGATTGGATATTGTCGTCCAGCAGATGTGAGAGGCTGAAAGCACTAAAAACCCAAAACGATGTGGGGGCTTTGGTCGTGGCCGCGGCCGCCCCCGCca < 2:374497/138‑1 (MQ=255) aaaaaTTCTGACTGAAGCAGCGGATAAACCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCAAAAATCCCAAACGATGTGCGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCca < 1:492961/138‑1 (MQ=255) aggagcGTATAAACCGCCAGAATGGGCGATTGGATTTTGTCTTGCAGGAGAGGTGAGAGGCTGAAAGCACCAAAAATCCCAAACGATGTTCGGGCTTTGGTCGTTGACGCGGCCGCGCCCGCCACAGGCTGGGgttgtt < 1:586168/136‑1 (MQ=255) agcagcGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGgttgtt > 2:125391/1‑139 (MQ=255) ggATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACAAAACGATGTTCGGGCTTTGGTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCa < 1:341468/139‑1 (MQ=255) gATAAATCGCCAGAATCAGCGATTGGATCATGTCTTTCAGCAGATGTGAGAGGCTGATAGCACCAAAAATACCAACCGATTTTCGGGCTTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCAt < 1:53066/139‑1 (MQ=255) tGTCTTTCAGCAGATTGGAGAGGCTGATAGCCCAAAAAACACCAAACGATGTCGGGGCTTTGGTCGTGGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTg < 1:593698/139‑1 (MQ=255) gtcttacagaagATGTGAGGGGCTGAAAGCCCCTAAAAACCCAAACGGTGTGCGGGCTTTGGTCTTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGc < 2:467708/133‑1 (MQ=255) ttCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGCTGTTCGGGCCTTGGTCGTTGCCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATg > 1:253187/1‑139 (MQ=255) agGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTa > 2:80935/1‑139 (MQ=255) aaaCGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTaa > 1:427915/1‑138 (MQ=255) | TCATGCCAATCTGCATAAATGTCAGAGAAAATTCTGACTGAAGCAGCGGATAAATCGCCAGAATCAGCGATTGGATCATGTCGTTCAGCAGATGTGAGAGGCTGATAGCACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAA > NZ_CP009273/499960‑500218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |