Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3410GA51.8% 7.4 / 36.3 27intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base G (4/9);  new base A (14/0);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 1.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT  >  NZ_CP009273/2380231‑2380478
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aTACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                <  1:402174/138‑1 (MQ=255)
      cagacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGtttt                                                                                                         <  2:357240/139‑1 (MQ=255)
        gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACAtt                                                                                                                                    >  2:320967/1‑109 (MQ=255)
        gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATg                                                                                                                                    <  1:320967/110‑1 (MQ=255)
         acagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACCGCAAGCACATTTTTTTTTTCCTCCACCGttttta                                                                                                       >  2:402174/1‑136 (MQ=255)
             aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGc                                                                                                                                   <  1:49813/106‑1 (MQ=255)
             aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGc                                                                                                                                   >  2:49813/1‑106 (MQ=255)
                       cAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGGAACATGa                                                                                         >  1:380630/1‑138 (MQ=255)
                                    aGGTAGTCGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGTCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCGCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg                                                                            <  2:412728/138‑1 (MQ=255)
                                         gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAAAAAAAACAACATGCAAATTTTATTTTTCCTCATCTGTTTTTAGATGGGAAACATGAAAATTAAAGGAGGgaaaa                                                                       >  2:317648/1‑138 (MQ=255)
                                                gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAGAAGCGGAACAATAAATTTAACGGAGGAAAAATAAATtgg                                                               >  1:342737/1‑139 (MQ=255)
                                                gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAACATAAAAATTAAAGGGGGGAAAAAAAATggg                                                               >  2:335007/1‑139 (MQ=255)
                                                 tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAAAGCACATTTTATTTTTCCTCCTATTGTTTAAGAAGCGAAAAATAAAAATTAAAGGGGGAAAAAAAAATggg                                                               >  1:100015/1‑138 (MQ=255)
                                                 tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTATTTTTCCTCATCTGTTTTAAGATGGGAAACATGAAAATCAAAGGGGGGAAAATAAATggg                                                               >  1:299808/1‑138 (MQ=255)
                                                           ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGGAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAACATTAAATTTAACGGAGGGAAAATAAATTGGGATGATTgggt                                                    >  2:80006/1‑137 (MQ=255)
                                                           ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGGCATTCCCAATTTATTTTTCCTCACCTGTTTTTAAATGCGAAACCAGAAATTCAAACGGGGAAAAATAAAAGGGGATGGTTTGCt                                                    >  1:445839/1‑139 (MQ=255)
                                                                        gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAt                                       <  1:335007/139‑1 (MQ=255)
                                                                         tAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGAACATTTTTTTTTTCCTCAACTGTTTTTAGATGCGGAAAATGAAATTTAACGGAGGGAAAAAAAAAGGGGATGATTTGCTATTTTGCAGAAAtt                                      >  1:312782/1‑139 (MQ=255)
                                                                           aataatAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACAATTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAAAAAGAAATTCAAAGGAGGAAAAATAAATGGGGGTGATTGGCTTTTTTTTAGAAAtag                                     >  2:353274/1‑136 (MQ=255)
                                                                           aataatAAAAATTTCTCGAGGCGGGGCCCGAAAAAAACACAAGAAAATTTTTTTTTTTCCCATCTGGTTTTAAATTGGAAACAATAAAATACACGGAGGAAAAATAAATGGGGAAGATTTGCAATTTTTTAGAAAATg                                     >  2:178805/1‑138 (MQ=255)
                                                                              aataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTAAGATGCGAAAAATGAAATTCAAAGGAGGGAAAAAAAATGGGGATGATTGGGTATTTTGCAGAAATTGaat                                  >  1:349050/1‑136 (MQ=255)
                                                                                taaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCCTCTGTTTTTAGATGCGAAAAATGAAAATTAAAGGAGGGAAAAAAAATTGGAATGTTTTGCTATTTTTCAAAAAATTACt                                  >  2:107015/1‑136 (MQ=255)
                                                                                  aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa                              <  1:178805/138‑1 (MQ=255)
                                                                                  aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa                              <  2:366617/138‑1 (MQ=255)
                                                                                              ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCaa                 <  2:349050/139‑1 (MQ=255)
                                                                                                  ggTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGAAACATGAAATTCAACGGAGGGAAAATAAATGGGAATGATTGGCTATTTTTTAGAAATTTACTCCGAAAAAATTAAACAACTGc             >  2:8226/1‑139 (MQ=255)
                                                                                                             aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACt  >  2:494280/1‑139 (MQ=255)
                                                                                                              gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa         >  2:590512/1‑131 (MQ=255)
                                                                                                              gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa         <  1:590512/131‑1 (MQ=255)
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ATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACT  >  NZ_CP009273/2380231‑2380478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑