Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3620CT53.6% 8.8 / 37.0 28intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base C (2/11);  new base T (15/0);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 3.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-06
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATA  >  NZ_CP009273/2380231‑2380497
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aTACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                   <  1:402174/138‑1 (MQ=255)
      cagacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGtttt                                                                                                                            <  2:357240/139‑1 (MQ=255)
         acagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACCGCAAGCACATTTTTTTTTTCCTCCACCGttttta                                                                                                                          >  2:402174/1‑136 (MQ=255)
                       cAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGGAACATGa                                                                                                            >  1:380630/1‑138 (MQ=255)
                                    aGGTAGTCGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGTCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCGCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg                                                                                               <  2:412728/138‑1 (MQ=255)
                                         gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAAAAAAAACAACATGCAAATTTTATTTTTCCTCATCTGTTTTTAGATGGGAAACATGAAAATTAAAGGAGGgaaaa                                                                                          >  2:317648/1‑138 (MQ=255)
                                                gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAGAAGCGGAACAATAAATTTAACGGAGGAAAAATAAATtgg                                                                                  >  1:342737/1‑139 (MQ=255)
                                                gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAACATAAAAATTAAAGGGGGGAAAAAAAATggg                                                                                  >  2:335007/1‑139 (MQ=255)
                                                 tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAAAGCACATTTTATTTTTCCTCCTATTGTTTAAGAAGCGAAAAATAAAAATTAAAGGGGGAAAAAAAAATggg                                                                                  >  1:100015/1‑138 (MQ=255)
                                                 tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTATTTTTCCTCATCTGTTTTAAGATGGGAAACATGAAAATCAAAGGGGGGAAAATAAATggg                                                                                  >  1:299808/1‑138 (MQ=255)
                                                           ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGGAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAACATTAAATTTAACGGAGGGAAAATAAATTGGGATGATTgggt                                                                       >  2:80006/1‑137 (MQ=255)
                                                           ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGGCATTCCCAATTTATTTTTCCTCACCTGTTTTTAAATGCGAAACCAGAAATTCAAACGGGGAAAAATAAAAGGGGATGGTTTGCt                                                                       >  1:445839/1‑139 (MQ=255)
                                                                        gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAt                                                          <  1:335007/139‑1 (MQ=255)
                                                                         tAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGAACATTTTTTTTTTCCTCAACTGTTTTTAGATGCGGAAAATGAAATTTAACGGAGGGAAAAAAAAAGGGGATGATTTGCTATTTTGCAGAAAtt                                                         >  1:312782/1‑139 (MQ=255)
                                                                           aataatAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGCACAATTTTTTTTTCCTCCTCTGTTTTTAGATGCGAAAAAAGAAATTCAAAGGAGGAAAAATAAATGGGGGTGATTGGCTTTTTTTTAGAAAtag                                                        >  2:353274/1‑136 (MQ=255)
                                                                           aataatAAAAATTTCTCGAGGCGGGGCCCGAAAAAAACACAAGAAAATTTTTTTTTTTCCCATCTGGTTTTAAATTGGAAACAATAAAATACACGGAGGAAAAATAAATGGGGAAGATTTGCAATTTTTTAGAAAATg                                                        >  2:178805/1‑138 (MQ=255)
                                                                              aataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTAAGATGCGAAAAATGAAATTCAAAGGAGGGAAAAAAAATGGGGATGATTGGGTATTTTGCAGAAATTGaat                                                     >  1:349050/1‑136 (MQ=255)
                                                                                taaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCCTCTGTTTTTAGATGCGAAAAATGAAAATTAAAGGAGGGAAAAAAAATTGGAATGTTTTGCTATTTTTCAAAAAATTACt                                                     >  2:107015/1‑136 (MQ=255)
                                                                                  aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa                                                 <  2:366617/138‑1 (MQ=255)
                                                                                  aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa                                                 <  1:178805/138‑1 (MQ=255)
                                                                                              ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCaa                                    <  2:349050/139‑1 (MQ=255)
                                                                                                  ggTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGAAACATGAAATTCAACGGAGGGAAAATAAATGGGAATGATTGGCTATTTTTTAGAAATTTACTCCGAAAAAATTAAACAACTGc                                >  2:8226/1‑139 (MQ=255)
                                                                                                             aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACt                     >  2:494280/1‑139 (MQ=255)
                                                                                                              gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa                            <  1:590512/131‑1 (MQ=255)
                                                                                                              gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa                            >  2:590512/1‑131 (MQ=255)
                                                                                                                         attttattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTg         <  1:80006/139‑1 (MQ=255)
                                                                                                                               ttttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGa    <  1:554052/138‑1 (MQ=255)
                                                                                                                                 ttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGata  <  2:380630/138‑1 (MQ=255)
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ATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATA  >  NZ_CP009273/2380231‑2380497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑