| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,897,051 | 0 | C | A | 58.3% | 5.4 / 16.2 | 12 | intergenic (+318/+1055) | ygcE/queE | FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE |
| Reads supporting (aligned to +/- strand): ref base C (0/5); new base A (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGG > NZ_CP009273/2896915‑2897155 | tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑TTAAAACa > 1:365263/1‑139 (MQ=255) acTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑TAAAAACCCACAttttac > 2:158786/1‑135 (MQ=255) tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑TTAAAAAACTCATTTAACTCAaaaaaa > 2:550800/1‑138 (MQ=255) tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑TAAAAAAAACCATTAACCTCAAAAAAAAAAAAAAATTCCacaatt > 1:388757/1‑134 (MQ=255) aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAAc < 1:230867/139‑1 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaa < 2:365263/139‑1 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaa < 1:158786/138‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAata < 1:431287/139‑1 (MQ=255) cTATTCATCAACAGCGTATAGAGGCGGTTATT‑TAAAAACAATCATTTTACTCACAACAAAAAAAAAATTCCCCAATTTTTTATTTACCCATGAATAAAAAAATTTAAAAAAATAATTTTTTTTTTAaaaaaaaaaaaa > 2:229463/1‑134 (MQ=255) ttCATCAACAGCGTATAGAGGCGGTTATGTTTAAAAAACCCATT‑TCCCCAAAAAAAAAAAAAAAATCCAGATTTTTTTTTTTACCAAAGAAAAAAAAACATTAAAAAAATAATGTTTTTTTTAAATAAAAATTaaaaag > 2:66923/1‑135 (MQ=255) ttCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACa < 2:41064/137‑1 (MQ=255) ttCATCAACAGCGTATAGAGGCGGTTATG‑TAAAAACACCCATTTACCTCAAAAAAAAAACAAAACTCCAGATTTTTTTTTTTAACAAAGAAAAAAAAAATTTAAAAAAATAATGTTATTTTTAAAAAAAAAATaaaaag > 1:323208/1‑135 (MQ=255) | TATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑TAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGG > NZ_CP009273/2896915‑2897155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |