| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,199,794 | 0 | C | T | 55.0% | 5.3 / 24.2 | 20 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (1/8); new base T (11/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG > NZ_CP009273/3199674‑3199920 | tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCCGACAAATA‑aaaaaaat > 1:497544/1‑137 (MQ=255)tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGAcaaaaa‑caaaaaat > 2:539307/1‑137 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATA‑CAAAAAATgg > 2:166636/1‑139 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAA‑AAAAAAATg > 1:436007/1‑137 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAg < 1:302751/136‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGCCAAATA‑CAAAAAATGGAGTTGGCa > 1:228964/1‑139 (MQ=255) cTTATTTTCGCGTTTATTCACGAACTGTTAATTACATATTCACGGTTGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGa < 1:186164/138‑1 (MQ=255) cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGa < 1:396792/138‑1 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 2:113187/139‑1 (MQ=255) aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa < 1:539307/138‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGCATTTTTTCCGACAAATATCAAAAAC‑AGAGTTGCCATCCTGAGCGAAAGTAATAAAAAACCAGCAGCGAATAAGttttga > 1:65953/1‑133 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATA‑AAAAAAATGGAGTTGGCATGGTGAGCGAAAGTAAAAAGCAAAAGGCAGTGAATAAGGTGaac > 2:531177/1‑133 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAA‑CAAAAAATGGAGTTGCCATGGTGAGGGAAAGAAATAAAAAAAAAGCAGTGAAAAAATTGACAAAGATTGtg > 2:192241/1‑138 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCacac > 2:360974/1‑58 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAc < 1:360974/58‑1 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAA‑AAAAAACTGGGGGTGGCCTTATGGGCGAAAGTAAAAAAAAACAAGGAATGAAAAAATTGACAGAGATTGTCGCTAAc > 2:366787/1‑139 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAA‑CAAAAACTGGAGGTGCCCTGATGAGCGGAAGAAATAAACAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAAATTTACCCCCATGAtttt > 1:422435/1‑138 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCCGACAAATA‑CAAAAACAGGAGGTGCCACGATGAGGGGCAGCAATAAACAAAAAGCAGCGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGCCCTGAtttt > 1:124218/1‑138 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtga < 1:614051/139‑1 (MQ=255) cGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:155811/139‑1 (MQ=255) | TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATA‑CAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG > NZ_CP009273/3199674‑3199920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |