Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,158,126	T→C	noncoding (1540/1542 nt)	BW25113_RS20590 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,158,126	0	T	C	100.0%	110.0 / NA	31	noncoding (1540/1542 nt)	BW25113_RS20590	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

GCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATT  >  NZ_CP009273/4158012‑4158243                                                                                                                   |                                                                                                                      gCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGt                                                                                                <  2:60698/138‑1 (MQ=11)              gtagCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGa                                                                                  <  1:430974/139‑1 (MQ=17)               tagCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGAt                                                                                 >  1:505142/1‑139 (MQ=17)                 gCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGAtt                                                                                <  1:405/138‑1 (MQ=17)                  cTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGt                                                                              <  2:426896/139‑1 (MQ=17)                       ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGAt                                                                         <  2:547351/139‑1 (MQ=17)                           cGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGa                                                                      >  2:504840/1‑138 (MQ=21)                                              ttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGtt                                                   >  2:223965/1‑138 (MQ=25)                                                gtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTa                                                 <  1:504840/138‑1 (MQ=37)                                                      cATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGtt                                           <  1:320189/138‑1 (MQ=255)                                                          aCTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAg                                      <  1:223965/139‑1 (MQ=255)                                                                   aaGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTg                              <  1:456493/138‑1 (MQ=255)                                                                   aaGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTg                              >  1:493472/1‑138 (MQ=255)                                                                    aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGaa                            <  2:505142/139‑1 (MQ=255)                                                                    aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGaa                            <  1:229621/139‑1 (MQ=255)                                                                     gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAg                                      <  2:219464/128‑1 (MQ=255)                                                                     gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAg                                      >  1:219464/1‑128 (MQ=255)                                                                         tAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGaa                       >  2:510217/1‑139 (MQ=255)                                                                                       tAGGGGAACCTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCt         <  1:402134/139‑1 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGa                                                                                  >  1:457012/1‑58 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGa                                                                                  <  2:457012/58‑1 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGa                                                                <  1:366235/76‑1 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGa                                                                >  2:366235/1‑76 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCttt       <  2:159015/133‑1 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCttt       >  1:159015/1‑133 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTAtt  >  2:7751/1‑138 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTAtt  >  2:445239/1‑138 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTAtt  >  2:28056/1‑138 (MQ=255)                                                                                               ccTGCGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTAtt  >  2:247922/1‑138 (MQ=255)                                                                                                   cGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGtt             >  2:174703/1‑123 (MQ=255)                                                                                                   cGGTTGGATCACCTCCCTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGtt             <  1:174703/123‑1 (MQ=255)                                                                                                                   |                                                                                                                      GCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATT  >  NZ_CP009273/4158012‑4158243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑