Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,279	T→G	F153V (TTT→GTT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,279	0	T	G	100.0%	76.0 / NA	20	F153V (TTT→GTT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/13);  total (7/13)

TCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATG  >  NZ_CP009273/50166‑50412                                                                                                                  |                                                                                                                                      tCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAAtt                                                                                                              <  2:172391/139‑1 (MQ=255)    aCGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTg                                                                                                           >  2:233083/1‑139 (MQ=255)       cAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGtat                                                                                                        <  1:365397/139‑1 (MQ=255)               gAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAAttt                                                                                                 <  1:269143/138‑1 (MQ=255)                          ccATTTCCCGGATTTCGAGCCGGATAACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGcccc                                                                                     <  2:245843/139‑1 (MQ=255)                          ccATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGcc                                                                                     <  1:614830/139‑1 (MQ=255)                                           aGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTAAGGCTAGCGCCGGATGCGACGCCGGTcg                                                                    >  1:156394/1‑139 (MQ=255)                                                 aTGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAACGCCGGATGCGACGCCGGTCGCGTCtt                                                              >  1:22179/1‑139 (MQ=255)                                                        ggAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc                                                       <  1:233083/139‑1 (MQ=255)                                                        ggAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc                                                       <  2:36769/139‑1 (MQ=255)                                                         gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc                                                      <  2:156394/139‑1 (MQ=255)                                                            tCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTc                                                   <  2:22179/139‑1 (MQ=255)                                                                      gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAgg                                         <  2:276796/139‑1 (MQ=255)                                                                            tCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc                                                       >  1:16380/1‑119 (MQ=255)                                                                            tCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc                                                       <  2:16380/119‑1 (MQ=255)                                                                                                        caGCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATcc       <  2:453406/139‑1 (MQ=255)                                                                                                          gCTATTGCGTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAGCGCCCGGTGCGGCGCCGGTCGCGTCTTTTCCGGCCCTCCTATATCAGGCCGTGTTTAAGACGCCGCCGCCTCGCCCAAAACCtt     >  2:504894/1‑139 (MQ=255)                                                                                                             aTTGCGTTGAGATTCTGGAGCGGCGGAAATTTTGTATAGAATTTAAGGGTAGGGGCGGAGGCGGCGCCGGGCGGGTGTTTTTAGGAATTTCTTAATCAGGCTGTGGTTAAGCTGCCGCCGCCTCGCCCCAATccctttg  >  2:182678/1‑134 (MQ=255)                                                                                                               tGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAgg                                         <  2:240437/98‑1 (MQ=255)                                                                                                               tGCGTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAgg                                         >  1:240437/1‑98 (MQ=255)                                                                                                                  |                                                                                                                                      TCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCTTATG  >  NZ_CP009273/50166‑50412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑