Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,588	T→C	L71P (CTG→CCG)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,588	0	T	C	100.0%	83.4 / NA	23	L71P (CTG→CCG)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

TCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGC  >  NZ_CP009273/1613454‑1613693                                                                                                                                       |                                                                                                          tCGCCTGCTTAACGCGTATCTGGCTCCGTTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCTCCGCGTGTATGACTCCGGTTGAACTGAAAAAGGCATTGTCGGTCTACCTGAGAGCACCGAct                                                                                                       <  2:447308/139‑2 (MQ=255)   gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGAccc                                                                                                      <  2:359139/138‑1 (MQ=255)   gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGAccc                                                                                                      <  2:223105/138‑1 (MQ=255)            aCGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTgg                                                                                             <  1:464059/138‑1 (MQ=255)                 tATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGcc                                                                                       <  1:357791/139‑1 (MQ=255)                       tctcTGCTGGATATTGCCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTc                                                                                  <  2:546951/138‑1 (MQ=255)                       tctcCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTgg                                                                                    <  2:230780/136‑1 (MQ=255)                                gATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAgg                                                                          <  1:12578/137‑1 (MQ=255)                                            gCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTg                                                                                     <  1:259040/114‑1 (MQ=255)                                            gCACAGTTTAAGGGGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTg                                                                                     >  2:259040/1‑114 (MQ=255)                                                            tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGa                                            >  2:9541/1‑139 (MQ=255)                                                            tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGa                                            >  1:525127/1‑139 (MQ=255)                                                            tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGa                                            >  1:359239/1‑139 (MQ=255)                                                                     tCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAc                                           <  1:92710/131‑1 (MQ=255)                                                                     tCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAc                                           >  2:92710/1‑131 (MQ=255)                                                                     tCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGcgcg                                    <  1:123908/138‑1 (MQ=255)                                                                          tGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                                >  2:165340/1‑137 (MQ=255)                                                                          tGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                                <  1:165340/137‑1 (MQ=255)                                                                                             cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTgg                           <  2:329753/123‑1 (MQ=255)                                                                                             cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTgg                           >  1:329753/1‑123 (MQ=255)                                                                                               gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTa                         <  1:600792/123‑1 (MQ=255)                                                                                               gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTa                         >  2:600792/1‑123 (MQ=255)                                                                                                        aaaaaGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc  <  1:9541/137‑1 (MQ=255)                                                                                                                                       |                                                                                                          TCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGC  >  NZ_CP009273/1613454‑1613693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑