Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,062,410	G→T	P76Q (CCG→CAG)	yihQ ←	sulfoquinovosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,062,410	0	G	T	100.0%	103.8 / NA	26	P76Q (CCG→CAG)	yihQ	sulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/14);  total (12/14)

GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCT  >  NZ_CP009273/4062277‑4062535                                                                                                                                      |                                                                                                                              gcgcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGAc                                                                                                                          <  1:293421/139‑1 (MQ=255)                                tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAttt                                                                                           >  2:258263/1‑139 (MQ=255)                                                         gCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCt                                                                  <  2:27775/139‑1 (MQ=255)                                                           cccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTg                                                                 >  2:573406/1‑138 (MQ=255)                                                           cccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTg                                                                 >  2:367376/1‑138 (MQ=255)                                                                aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAAt                                                             <  1:258263/137‑1 (MQ=255)                                                                                   aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                         <  1:573406/138‑1 (MQ=255)                                                                                     cGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATcc                                      >  1:444995/1‑139 (MQ=255)                                                                                            cTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAc                               <  1:350439/139‑1 (MQ=255)                                                                                               aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAcc                              <  1:421333/137‑1 (MQ=255)                                                                                               aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCt                             >  1:394452/1‑138 (MQ=255)                                                                                                    aGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGcc                        >  2:379385/1‑138 (MQ=255)                                                                                                         cACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCa                  >  1:460299/1‑139 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCtt                                                                         <  1:487926/82‑1 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCtt                                                                         >  2:487926/1‑82 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGcc                        >  1:512777/1‑131 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGcc                        <  2:512777/131‑1 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAt                 >  2:302782/1‑138 (MQ=255)                                                                                                             gCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa              <  1:458590/139‑1 (MQ=255)                                                                                                             gCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa              <  1:426251/139‑1 (MQ=255)                                                                                                             gCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATaaa              <  2:460299/139‑1 (MQ=255)                                                                                                                       aTTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTAt    <  2:78101/139‑1 (MQ=255)                                                                                                                        ttAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATc   >  2:539966/1‑139 (MQ=255)                                                                                                                          aaccaaccATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt  <  2:444995/138‑1 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt  <  2:413951/132‑1 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCt  >  1:413951/1‑132 (MQ=255)                                                                                                                                      |                                                                                                                              GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCT  >  NZ_CP009273/4062277‑4062535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑