Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,659,452	(T)7→6	intergenic (+75/‑84)	dmsD → / → clcB	Tat proofreading chaperone DmsD/voltage‑gated ClC‑type chloride channel ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,659,446	0	T	.	100.0%	130.6 / NA	30	intergenic (+69/‑90)	dmsD/clcB	Tat proofreading chaperone DmsD/voltage‑gated ClC‑type chloride channel ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/16);  total (14/16)

AACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACT  >  NZ_CP009273/1659314‑1659571                                                                                                                                     |                                                                                                                              aaCGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttttt                                                                                                                          <  1:409193/138‑1 (MQ=255) aaCGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttttt                                                                                                                          <  1:470109/138‑1 (MQ=255)  aCGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttat                                                                                                                        <  1:467314/139‑3 (MQ=255)   cGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttatc                                                                                                                       <  2:28029/139‑4 (MQ=255)        gcgcAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCtt                                                                                                                  >  1:294573/1‑138 (MQ=255)              tGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAgcac                                                                                                           >  1:386938/1‑139 (MQ=255)                  aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttat                                                                                                                        >  1:109318/1‑121 (MQ=255)                  aaTCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttat                                                                                                                        <  2:109318/123‑3 (MQ=255)                    tCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTg                                                                                                     >  2:428909/1‑139 (MQ=255)                             ttAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                                                                                            <  2:184153/139‑1 (MQ=255)                                       tCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtt                                                                                  >  2:280486/1‑139 (MQ=255)                                                            gATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc                                                              <  1:428909/138‑1 (MQ=255)                                                                  aTCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt                                                        >  1:383437/1‑138 (MQ=255)                                                                  aTCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt                                                        >  1:93328/1‑138 (MQ=255)                                                                   tCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa                                                      <  2:93328/139‑1 (MQ=255)                                                                   tCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa                                                      <  2:294573/139‑1 (MQ=255)                                                                   tCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa                                                      <  1:335753/139‑1 (MQ=255)                                                                        gTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc                                                 <  1:280486/139‑1 (MQ=255)                                                                               tGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCt                                          >  2:492698/1‑139 (MQ=255)                                                                                 ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc                                        >  1:580814/1‑139 (MQ=255)                                                                                 ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc                                        >  1:459502/1‑139 (MQ=255)                                                                                 ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc                                        >  1:198907/1‑139 (MQ=255)                                                                                 ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc                                        >  1:252866/1‑139 (MQ=255)                                                                                     aTGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCAt                                    <  1:169588/139‑1 (MQ=255)                                                                                                  cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGttttttat                                                                                                                        >  1:546912/1‑41 (MQ=255)                                                                                                  cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGttttttat                                                                                                                        <  2:546912/43‑3 (MQ=255)                                                                                                  cgcgCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc                                                 >  1:16520/1‑113 (MQ=255)                                                                                                  cgcgCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc                                                 <  2:16520/113‑1 (MQ=255)                                                                                                  cgcgCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTacac                       <  2:252866/139‑1 (MQ=255)                                                                                                    cgcAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTTCTCATGCATCATCTAcacag                     <  2:561564/139‑2 (MQ=255)                                                                                                    cgcAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACAt                     <  2:292056/139‑1 (MQ=255)                                                                                                                   ataGGTTACAGTGGCACG‑TTTTGTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTgcg      <  2:184374/139‑1 (MQ=255)                                                                                                                      ggTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCa    <  2:198907/138‑1 (MQ=255)                                                                                                                       gTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc                                                                <  1:253405/77‑1 (MQ=255)                                                                                                                       gTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc                                                                >  2:253405/1‑77 (MQ=255)                                                                                                                       gTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACt  <  2:367384/139‑1 (MQ=255)                                                                                                                           cAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc                                           >  2:66862/1‑94 (MQ=255)                                                                                                                           cAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc                                           <  1:66862/94‑1 (MQ=255)                                                                                                                                     |                                                                                                                              AACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACT  >  NZ_CP009273/1659314‑1659571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑