Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,732,351	(A)7→6	coding (251/1026 nt)	purR →	HTH‑type transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,732,345	0	A	.	100.0%	148.6 / NA	34	coding (245/1026 nt)	purR	HTH‑type transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/17);  total (17/17)

GGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGA  >  NZ_CP009273/1732210‑1732474                                                                                                                                        |                                                                                                                                  gggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaa                                                                                                                                 >  1:403055/1‑138 (MQ=255)       cGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaaaaattgc                                                                                                                          <  1:346854/139‑5 (MQ=255)               gAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaa                                                                                                                 <  1:341343/139‑1 (MQ=255)                     cacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           <  2:291869/139‑1 (MQ=255)                          ctCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      >  2:319267/1‑139 (MQ=255)                                  gcgcGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTg                                                                                              >  2:280573/1‑139 (MQ=255)                                  gcgcGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTg                                                                                              >  2:218705/1‑139 (MQ=255)                                    gcgGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      >  1:272005/1‑129 (MQ=255)                                    gcgGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACAccc                                                                                                      <  2:272005/129‑1 (MQ=255)                                      ggTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  1:431487/139‑1 (MQ=255)                                      ggTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  2:140070/139‑1 (MQ=255)                                       gTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCaa                                                                                         >  2:121253/1‑139 (MQ=255)                                             agTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTg                                                                                   >  1:168322/2‑139 (MQ=255)                                                aGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGaa                                                                                >  1:428344/1‑139 (MQ=255)                                                 gCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGaa                                                                                <  2:475520/138‑1 (MQ=255)                                                               cacaCCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACa                                                                 <  2:3151/139‑1 (MQ=255)                                                                   ccAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  2:331931/110‑1 (MQ=255)                                                                   ccAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          >  1:331931/1‑110 (MQ=255)                                                                       gTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTTCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGTTTACACCCTGATTCTGGGCa                                                                                          >  1:519200/1‑106 (MQ=255)                                                                       gTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTTCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                          <  2:519200/106‑1 (MQ=255)                                                                            tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACa                                                                 >  2:39881/1‑126 (MQ=255)                                                                            tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACa                                                                 <  1:39881/126‑1 (MQ=255)                                                                            tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTAt                                                       <  2:150851/136‑1 (MQ=255)                                                                            tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTg                                                    >  1:9164/1‑139 (MQ=255)                                                                                 ttGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCgat                                               >  2:21354/1‑139 (MQ=255)                                                                                  tGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTACCTGTCgat                                               >  2:131429/1‑138 (MQ=255)                                                                                  tGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCGGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCgatg                                              >  1:481594/1‑139 (MQ=255)                                                                                              gcagcGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCaaaaa                                  <  2:428344/139‑1 (MQ=255)                                                                                              gcagcGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCaaaaa                                  <  2:403055/139‑1 (MQ=255)                                                                                                    aaGCGGCCTATTTTGCCGAGCTCATTGAAGCAGTTG‑AAAAAATTGCTTACAGAAAGGttt                                                                                                           <  2:81959/60‑2 (MQ=255)                                                                                                    aaGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTa                                                                                                           >  1:81959/1‑60 (MQ=255)                                                                                                            tATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTct                    >  1:491624/1‑139 (MQ=255)                                                                                                                 tGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTctgctg                <  2:57041/138‑1 (MQ=255)                                                                                                                   ccGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGt              >  2:347069/1‑138 (MQ=255)                                                                                                                               aaGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGa  <  2:491624/138‑1 (MQ=255)                                                                                                                               aaGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGa  <  1:21354/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGA  >  NZ_CP009273/1732210‑1732474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑