Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,891,886	Δ1 bp	coding (698/1365 nt)	sdaA →	L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,891,884	0	C	.	100.0%	57.2 / NA	14	coding (696/1365 nt)	sdaA	L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/6);  total (8/6)

GCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NZ_CP009273/1891749‑1892005                                                                                                                                        |                                                                                                                          gCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGccg                                                                                                                         <  1:181466/138‑2 (MQ=255)              gCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGcc                                                                                                             <  2:34435/136‑1 (MQ=255)                                      cacaGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGAt                                                                                  >  1:136871/1‑139 (MQ=255)                                           cAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTgg                                                                             >  1:565202/1‑139 (MQ=255)                                                aagaGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTc                                                                         >  1:150711/1‑138 (MQ=255)                                                   agaTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGc                                                                     >  1:117183/1‑139 (MQ=255)                                                     aTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCa                                                                       >  1:484086/1‑135 (MQ=255)                                                     aTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCa                                                                       <  2:484086/135‑1 (MQ=255)                                                         acgaGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGAGCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATaaa                                                               <  2:168319/139‑1 (MQ=255)                                                                tttCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTa                                                        <  2:500941/139‑1 (MQ=255)                                                                                      aTGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGa                                  <  2:417483/139‑1 (MQ=255)                                                                                      aTGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGa                                  <  2:494284/139‑1 (MQ=255)                                                                                                 tATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACa                       >  2:160533/1‑139 (MQ=255)                                                                                                      atcgCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGttt                  >  1:314374/1‑139 (MQ=255)                                                                                                                        aaGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACg  >  2:43151/1‑137 (MQ=255)                                                                                                                                        |                                                                                                                          GCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NZ_CP009273/1891749‑1892005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑