Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	475,405	Δ1 bp	intergenic (‑31/+141)	maa ← / ← hha	maltose O‑acetyltransferase/HHA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	475,404	0	A	.	100.0%	99.6 / NA	23	intergenic (‑30/+142)	maa/hha	maltose O‑acetyltransferase/HHA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/14);  total (9/14)

GTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCAACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCATGGCGAAAAAGTATAAAATTCTTAATAAACAGCCGG  >  NZ_CP009273/475270‑475519                                                                                                                                       |                                                                                                                    gTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGAACTCCGGTTGCTGAAAATGCGGCaca                                                                                                                   >  1:542266/1‑135 (MQ=255)          aaTAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGa                                                                                                       <  2:273427/139‑1 (MQ=255)          aaTAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGa                                                                                                       <  2:61791/139‑1 (MQ=255)                cTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTc                                                                                                 <  2:182240/139‑1 (MQ=255)                    cGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGt                                                                                              >  1:267680/1‑138 (MQ=255)                        cgcgcAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGaa                                                                                           >  1:54964/1‑137 (MQ=255)                             aGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCaca                                                                                                                   <  2:555647/109‑3 (MQ=255)                             aGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCaca                                                                                                                   >  1:555647/1‑107 (MQ=255)                                  aTCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTcc                                                                                >  1:332955/1‑138 (MQ=255)                                         gaTAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACAt                                                                        <  2:54964/139‑1 (MQ=255)                                         gaTAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACAt                                                                        <  2:111710/139‑1 (MQ=255)                                                 ctcATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGa                                                                >  2:321910/1‑139 (MQ=255)                                                    aTCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGAt                                                               <  1:471535/137‑1 (MQ=255)                                                    aTCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATa                                                              >  1:413751/1‑138 (MQ=255)                                                    aTCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATa                                                              >  2:313406/1‑138 (MQ=255)                                                         ccGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGAC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAg                                                           <  1:56898/136‑1 (MQ=255)                                                            aGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATcaca                                                     <  2:259870/139‑1 (MQ=255)                                                            aGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATcaca                                                     <  1:422463/139‑1 (MQ=255)                                                               gATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAg                                                  <  2:578413/139‑1 (MQ=255)                                                               gATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAAAACGTTCCCATTACATCTGTGTGATAAAGATCACATAg                                                  <  2:267680/139‑1 (MQ=255)                                                                           cAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCa                                                                                                                     >  2:327617/1‑61 (MQ=255)                                                                           cAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCa                                                                                                                     <  1:327617/61‑1 (MQ=255)                                                                           cAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCa                                      >  2:516876/1‑139 (MQ=255)                                                                                   atcTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCATGGCGaaa                              <  1:516876/139‑1 (MQ=255)                                                                                   atcTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCATGGCGaaa                              <  2:413751/139‑1 (MQ=255)                                                                                                               aCCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGCACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCATGGCGAAAAAGTATAAAATTCTTAATAAACAGCCgg  >  2:324940/1‑139 (MQ=255)                                                                                                                    cGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAg                                                  >  2:312463/1‑86 (MQ=255)                                                                                                                    cGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAg                                                  <  1:312463/86‑1 (MQ=255)                                                                                                                                       |                                                                                                                    GTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCAACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCATGGCGAAAAAGTATAAAATTCTTAATAAACAGCCGG  >  NZ_CP009273/475270‑475519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑