Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	481,730	Δ1 bp	coding (514/648 nt)	acrR →	multidrug efflux transporter transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	481,728	0	T	.	100.0%	108.7 / NA	25	coding (512/648 nt)	acrR	multidrug efflux transporter transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/9);  total (16/9)

GCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACG  >  NZ_CP009273/481601‑481859                                                                                                                                |                                                                                                                                    gCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGAt                                                                                                                         >  1:161031/1‑139 (MQ=255)                 gtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCttt                                                                                                         >  2:152781/1‑139 (MQ=255)                          gTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                                                                >  1:511251/1‑139 (MQ=255)                                              aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGAt                                                                                       >  1:202532/1‑128 (MQ=255)                                              aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGAt                                                                                       <  2:202532/128‑1 (MQ=255)                                              aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaag                                                                             >  2:380646/1‑138 (MQ=255)                                              aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaag                                                                             >  2:302326/1‑138 (MQ=255)                                              aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaag                                                                             >  2:285455/1‑138 (MQ=255)                                                gTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaag                                                                          >  2:68369/1‑139 (MQ=255)                                                gTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaag                                                                          >  2:573776/1‑139 (MQ=255)                                                          tGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTa                                                                >  2:35839/1‑139 (MQ=255)                                                                      aaaaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt                                                     >  2:170315/1‑138 (MQ=255)                                                                      aaaaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt                                                     >  2:44524/1‑138 (MQ=255)                                                                            ttGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGga                                              <  1:68369/139‑1 (MQ=255)                                                                                         tAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCt                                  <  1:573776/138‑1 (MQ=255)                                                                                         tAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCt                                  <  2:435396/138‑1 (MQ=255)                                                                                                gcgTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCCCCTGTGCCCCa                          >  2:175170/1‑139 (MQ=255)                                                                                                 cgTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCAc                         <  1:44524/139‑1 (MQ=255)                                                                                                  gTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACg                        <  1:421632/139‑1 (MQ=255)                                                                                                     gcgcAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCtt                     <  1:152781/139‑1 (MQ=255)                                                                                                                   atGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGga                                              <  2:270372/100‑1 (MQ=255)                                                                                                                   atGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGga                                              >  1:270372/1‑100 (MQ=255)                                                                                                                     gacaggCTATA‑TTCCGTCCTGATGGAAACCTGTCTCTTGGCCCCGCAATCTTTTGATCTTAAAAAAAAAGCCCGCGATTACGTGCCCATTTTACTGGAGATTTATCTCCTGTGCCCCACGCTTGGTAATCCTGCCACTa     <  2:64623/135‑1 (MQ=255)                                                                                                                        cgGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAAc   >  1:353027/1‑138 (MQ=255)                                                                                                                         ggCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACg  >  2:474581/1‑138 (MQ=255)                                                                                                                                |                                                                                                                                    GCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACG  >  NZ_CP009273/481601‑481859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑