Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	967,323	Δ1 bp	coding (116/894 nt)	ycbJ →	YcbJ family phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	967,321	0	A	.	100.0%	181.6 / NA	41	coding (114/894 nt)	ycbJ	YcbJ family phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/21);  total (20/21)

GTAGCTTTTATGAGGGTAATCTGAATGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGGAAACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAG  >  NZ_CP009273/967184‑967454                                                                                                                                          |                                                                                                                                      gTAGCTTTTATGAGGGTAATCTGAATGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGGaa                                                                                                                                      >  2:270639/1‑139 (MQ=255)                    tCTGAATGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTTGGCTTTGTATGACAGCCAGGG‑AACCCAATGCAGTTAATGGc                                                                                                                   >  2:368395/1‑138 (MQ=255)                        aaTGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTTTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCaag                                                                                                                <  1:344475/137‑1 (MQ=255)                        aaTGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCaag                                                                                                                <  2:581648/137‑1 (MQ=255)                             aaCAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCtt                                                                                                          >  1:22330/1‑138 (MQ=255)                             aaCAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCtt                                                                                                          >  2:70347/1‑138 (MQ=255)                               cAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAg                                                                                                       >  2:470534/1‑139 (MQ=255)                                aGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAg                                                                                                       >  1:408716/1‑138 (MQ=255)                                  cTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTAc                                                                                                    >  1:415702/1‑139 (MQ=255)                                  cTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTAc                                                                                                    >  1:297306/1‑139 (MQ=255)                                     cGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGcc                                                                                                 >  1:412592/1‑139 (MQ=255)                                       tGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGc                                                                                                  >  2:313988/1‑136 (MQ=255)                                       tGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGc                                                                                                  <  1:313988/136‑1 (MQ=255)                                          cGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGa                                                                                             >  2:315849/1‑138 (MQ=255)                                                      tttACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAAc                                                                                >  2:230335/1‑139 (MQ=255)                                                             ggCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCAt                                                                          <  2:22330/138‑1 (MQ=255)                                                             ggCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATg                                                                         >  1:223916/1‑139 (MQ=255)                                                                                ttGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTa                                                                                                                        <  1:395560/73‑1 (MQ=255)                                                                                ttGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTa                                                                                                                        >  2:395560/1‑73 (MQ=255)                                                                                ttGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCa                                                      <  2:297306/139‑1 (MQ=255)                                                                                ttGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCa                                                      <  1:154404/139‑1 (MQ=255)                                                                                ttGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCa                                                      <  1:304882/139‑1 (MQ=255)                                                                                     tGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCaagaag                                                 >  1:136695/1‑139 (MQ=255)                                                                                     tGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCaagaag                                                 <  2:223916/139‑1 (MQ=255)                                                                                         tCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTgg                                              >  1:69574/1‑138 (MQ=255)                                                                                           aaTGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGa                                            <  1:470534/138‑1 (MQ=255)                                                                                           aaTGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGAc                                           <  1:70347/139‑1 (MQ=255)                                                                                           aaTGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGAc                                           >  1:107471/1‑139 (MQ=255)                                                                                           aaTGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGAc                                           <  1:230335/139‑1 (MQ=255)                                                                                           aaTGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGAc                                           <  2:8955/139‑1 (MQ=255)                                                                                                 aaaGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCg                                     >  2:113722/1‑139 (MQ=255)                                                                                                        aTACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCg                              <  2:107471/139‑1 (MQ=255)                                                                                                         tACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGa                             <  2:408716/139‑1 (MQ=255)                                                                                                         tACGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGa                             >  2:554762/1‑139 (MQ=255)                                                                                                           cGGCGTTGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGAc                            <  1:554762/138‑1 (MQ=255)                                                                                                                ttGTGGGCTTTGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTa                      <  2:139094/139‑1 (MQ=255)                                                                                                                          tGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGtgat             <  1:270639/138‑1 (MQ=255)                                                                                                                          tGTATGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGtgat             >  1:13158/1‑138 (MQ=255)                                                                                                                              tGACAGCCAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGc        <  2:69574/139‑1 (MQ=255)                                                                                                                                    ccAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCg                              >  2:267447/1‑111 (MQ=255)                                                                                                                                    ccAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCg                              <  1:267447/111‑1 (MQ=255)                                                                                                                                    ccAGGG‑AACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATgaag  <  2:136695/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GTAGCTTTTATGAGGGTAATCTGAATGGAACAGCTGCGTGCCGAATTAAGCCATTTACTGGGCGAAAAACTCAGTCGTATTGAGTGCGTCAATGAAAAAGCGGATACGGCGTTGTGGGCTTTGTATGACAGCCAGGGAAACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAG  >  NZ_CP009273/967184‑967454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑