Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,292,953	+C	coding (322/444 nt)	yhbP ←	YhbP family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,292,952	1	.	C	100.0%	141.6 / NA	41	A108G (GCC→GGC)	yhbP	YhbP family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/21);  total (20/21)

CGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGG  >  NZ_CP009273/3292824‑3293084                                                                                                                                  |                                                                                                                                     cgAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGa                                                                                                                              >  2:79844/1‑138 (MQ=255)      cGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcga                                                                                                                        <  2:457383/139‑1 (MQ=255)          aCGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgacga                                                                                                                     <  1:218275/138‑1 (MQ=255)                gCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACg                                                                                                              >  1:206298/1‑139 (MQ=255)                gCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACg                                                                                                              >  1:292951/1‑139 (MQ=255)                       gCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGAAAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTgcg                                                                                                       >  2:394034/1‑139 (MQ=255)                            aGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTgcgcgc                                                                                                    >  2:303307/1‑137 (MQ=255)                            aGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTgcgcgc                                                                                                    >  1:144126/1‑137 (MQ=255)                            aGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGcgcgcg                                                                                                   <  1:260817/138‑1 (MQ=255)                                gcgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:422135/1‑139 (MQ=255)                                gcgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:324560/1‑139 (MQ=255)                                gcgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:31912/1‑139 (MQ=255)                                gcgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTc                                                                                              >  1:184741/1‑139 (MQ=255)                                 cgTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCg                                                                                             <  1:303307/139‑1 (MQ=255)                                  gTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGc                                                                                            >  2:264815/1‑139 (MQ=255)                                  gTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGAAAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGc                                                                                            <  1:394034/139‑1 (MQ=255)                                        aaaTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCt                                                                                      <  2:324560/139‑1 (MQ=255)                                                             ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  1:344301/139‑1 (MQ=255)                                                             ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  2:206298/139‑1 (MQ=255)                                                             ccAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                                 <  2:184741/139‑1 (MQ=255)                                                                  gtgtTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                                            <  2:31912/139‑1 (MQ=255)                                                                     ttgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCttt                                                          <  2:144126/138‑1 (MQ=255)                                                                     ttgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTa                                                         <  1:344240/139‑1 (MQ=255)                                                                      tgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTa                                                         >  1:30780/1‑138 (MQ=255)                                                                             gAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAt                                                                   <  2:264032/121‑1 (MQ=255)                                                                             gAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAt                                                                   >  1:264032/1‑121 (MQ=255)                                                                              aaTTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGcaca                                                >  1:351465/1‑139 (MQ=255)                                                                                  tGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcg                                             <  2:422135/138‑1 (MQ=255)                                                                                  tGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcg                                             <  2:47237/138‑1 (MQ=255)                                                                                            gAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACg                                   <  2:342724/138‑1 (MQ=255)                                                                                              gCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTa                                <  2:30780/139‑1 (MQ=255)                                                                                               ccGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTAc                               >  2:169637/1‑139 (MQ=255)                                                                                                         cacaCCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGc                     <  2:348766/139‑1 (MQ=255)                                                                                                          acacCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCt                    <  1:513580/139‑1 (MQ=255)                                                                                                                gggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGt              >  2:557950/1‑139 (MQ=255)                                                                                                                 ggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTc                                                                                     >  2:407984/1‑67 (MQ=255)                                                                                                                 ggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTc                                                                                     <  1:407984/67‑1 (MQ=255)                                                                                                                 ggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt             >  1:43804/1‑139 (MQ=255)                                                                                                                 ggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGtt             >  1:316241/1‑139 (MQ=255)                                                                                                                      aCAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCg        >  2:86772/1‑139 (MQ=255)                                                                                                                            ttCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCgg  <  1:50696/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTCCGG  >  NZ_CP009273/3292824‑3293084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑