Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273341,9440TC56.1% 2.7 / 83.6 57G35G (GGA→GGG) yahNLysE family transporter
Reads supporting (aligned to +/- strand):  ref base T (11/14);  new base C (15/17);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAG  >  NZ_CP009273/341808‑342078
                                                                                                                                        |                                                                                                                                      
gCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGcccc                                                                                                                                      >  1:47998/1‑139 (MQ=255)
gCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGcccc                                                                                                                                      >  1:358770/1‑139 (MQ=255)
gCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTcc                                                                                                                                      >  1:191535/1‑139 (MQ=255)
 cGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCcccc                                                                                                                                     >  1:573675/1‑139 (MQ=255)
  gTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCg                                                                                                                                    >  2:187567/1‑139 (MQ=255)
         gCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTaa                                                                                                                              >  1:466111/1‑138 (MQ=255)
           gTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTaaaa                                                                                                                            >  2:135672/1‑138 (MQ=255)
           gTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTaaaa                                                                                                                            >  2:483918/1‑138 (MQ=255)
               cAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAg                                                                                                                        <  2:533365/138‑1 (MQ=255)
                        acaaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  1:568958/139‑1 (MQ=255)
                        acaaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  1:483918/139‑1 (MQ=255)
                        acaaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCAGGATTAAAAAAAGTAATCACGaa                                                                                                              <  2:20180/139‑1 (MQ=255)
                         caaacCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGCTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAAc                                                                                                             <  2:235741/139‑1 (MQ=255)
                               caacccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCt                                                                                                       <  2:466111/139‑1 (MQ=255)
                                  cccGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACg                                                                                                    <  2:358770/139‑1 (MQ=255)
                                    cGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  <  1:537360/139‑1 (MQ=255)
                                    cGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  <  2:86398/139‑1 (MQ=255)
                                    cGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  <  2:47998/139‑1 (MQ=255)
                                      gAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAg                                                                                                                        <  2:117436/115‑1 (MQ=255)
                                      gAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAg                                                                                                                        >  1:117436/1‑115 (MQ=255)
                                                 aTCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAg                                                                                                                        >  2:340895/1‑104 (MQ=255)
                                                 aTCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAg                                                                                                                        <  1:340895/104‑1 (MQ=255)
                                                 aTCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  <  2:545647/126‑1 (MQ=255)
                                                 aTCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  >  1:545647/1‑126 (MQ=255)
                                                 aTCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCa                                                                                     >  1:441994/1‑139 (MQ=255)
                                                      ccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCaa                                                                                <  1:154570/139‑1 (MQ=255)
                                                      ccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCaa                                                                                <  1:187567/139‑1 (MQ=255)
                                                               cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              >  1:111771/1‑100 (MQ=255)
                                                               cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  2:111771/100‑1 (MQ=255)
                                                                    aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              >  1:374887/1‑95 (MQ=255)
                                                                    aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  2:374887/95‑1 (MQ=255)
                                                                    aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGa                                                                  >  1:66039/1‑139 (MQ=255)
                                                                     gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAt                                                                 >  2:293498/1‑139 (MQ=255)
                                                                       ccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAttt                                                               <  1:360220/139‑1 (MQ=255)
                                                                         ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              >  1:31410/1‑90 (MQ=255)
                                                                         ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  2:31410/90‑1 (MQ=255)
                                                                          gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCAt                                                            <  1:305626/139‑1 (MQ=255)
                                                                          gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCAt                                                            <  2:66039/139‑1 (MQ=255)
                                                                          gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  >  2:526737/1‑101 (MQ=255)
                                                                          gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGt                                                                                                  <  1:526737/101‑1 (MQ=255)
                                                                                    cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGa                                                                            <  1:74579/113‑1 (MQ=255)
                                                                                    cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGa                                                                            >  2:74579/1‑113 (MQ=255)
                                                                                    cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaataa                                                  <  1:204785/139‑1 (MQ=255)
                                                                                    cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaat                                                    >  2:204785/1‑137 (MQ=255)
                                                                                    cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCataaataa                                                  <  2:441994/139‑1 (MQ=255)
                                                                                            cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaa                                                                                                                             <  1:414035/56‑1 (MQ=255)
                                                                                            cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTaaa                                                                                                                             >  2:414035/1‑56 (MQ=255)
                                                                                            cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                           >  2:319530/1‑138 (MQ=255)
                                                                                                              gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAc                        >  1:122804/1‑139 (MQ=255)
                                                                                                              gTTTGTACTACCACAAAGAGATTGGCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAc                        >  1:159198/1‑139 (MQ=255)
                                                                                                                 tGTACTACCACACAGAGATTGGCCCCCGGATTAAGACAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAccc                      >  2:207078/1‑137 (MQ=255)
                                                                                                                   tactacCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaaa                    >  1:46504/1‑138 (MQ=255)
                                                                                                                        ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                         >  1:411380/1‑112 (MQ=255)
                                                                                                                        ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                         <  2:411380/112‑1 (MQ=255)
                                                                                                                         cacaAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc             <  2:191535/139‑1 (MQ=255)
                                                                                                                                agaTTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              >  2:290359/1‑35 (MQ=255)
                                                                                                                                agaTTGGCCCCCGGATTAAAAAAAGTAATCACGaa                                                                                                              <  1:290359/35‑1 (MQ=255)
                                                                                                                                  aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAtt                                                                <  2:405059/79‑1 (MQ=255)
                                                                                                                                  aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGAtt                                                                >  1:405059/1‑79 (MQ=255)
                                                                                                                                     ggCCCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTACATCATATTTCTACCAAAAATAATCGGGTGCgagag  <  1:135672/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCGTAATTAGCGTTGCAAGACCAAACAAACCCAACCCGGAATAAAATGCATCGCCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAG  >  NZ_CP009273/341808‑342078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑