| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 641,227 | 0 | T | C | 70.5% | 53.4 / 38.0 | 44 | A270A (GCA→GCG) | citT | citrate/succinate antiporter CitT |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base C (16/15); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAAT > NZ_CP009273/641091‑641361 | gcagACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGc < 2:442262/139‑1 (MQ=255) agACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCa < 2:461213/139‑1 (MQ=255) agACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCa < 1:182703/139‑1 (MQ=255) ccAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCActt < 1:433034/139‑1 (MQ=255) cGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCa < 1:481062/139‑1 (MQ=255) aTGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGAttt < 1:102536/139‑1 (MQ=255) cTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCgg < 1:487715/139‑1 (MQ=255) cACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGt < 2:308317/137‑1 (MQ=255) cACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGt < 1:269350/137‑1 (MQ=255) ccAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTa < 1:171740/137‑1 (MQ=255) ccAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGAGTGATTTCCGGTTTGTa > 2:254456/1‑137 (MQ=255) aTACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCAc < 1:6606/139‑1 (MQ=255) acaAACCTAAGCTGAGCACTACAAGGCCAATCAGTGTCGACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAgg > 2:263335/1‑138 (MQ=255) aGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCTCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCaa > 2:84642/1‑139 (MQ=255) aGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCaa > 1:96206/1‑139 (MQ=255) aGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCaa > 2:544370/1‑139 (MQ=255) cAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAAt > 1:409172/1‑138 (MQ=255) tACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGc > 2:532688/1‑75 (MQ=255) tACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGc < 1:532688/75‑1 (MQ=255) ggCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGc > 1:234517/1‑137 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGAttt < 2:262609/88‑1 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGAttt > 1:262609/1‑88 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATaa > 2:577765/1‑139 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATaa > 1:51210/1‑139 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATaa > 1:279078/1‑139 (MQ=255) aTCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATaa > 1:425511/1‑139 (MQ=255) tCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAAc < 1:279713/139‑1 (MQ=255) tCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCa < 1:401543/81‑1 (MQ=255) tCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCa > 2:401543/1‑81 (MQ=255) aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTa > 1:242745/1‑74 (MQ=255) aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTa < 2:242745/74‑1 (MQ=255) aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAgg > 1:273794/1‑138 (MQ=255) aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCActtctt < 2:34290/49‑1 (MQ=255) aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCActtctt > 1:34290/1‑49 (MQ=255) gcgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGaa < 2:273794/139‑1 (MQ=255) gcgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGaa < 2:57156/139‑1 (MQ=255) cgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAAc > 1:69845/1‑139 (MQ=255) cgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAAc > 1:213499/1‑139 (MQ=255) ccATGGTTTTCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTg > 2:4663/1‑139 (MQ=255) ttCAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAg < 2:409172/138‑1 (MQ=255) cAGTTCATCACCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCt < 1:84642/138‑1 (MQ=255) tcaCCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGa < 2:234517/139‑1 (MQ=255) tcaCCCGCCCAGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGa < 2:69845/139‑1 (MQ=255) aGGTCGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAAt > 1:529621/1‑139 (MQ=255) | GCAGACCAACCGCAGTAGCATTAATGACTTCACTGCCAAATACCCACAAACCTAAGCTGAGCAATACAAGGCCAATCAGTGTCCACTCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACCGGCAAT > NZ_CP009273/641091‑641361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |