| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 982,950 | 0 | T | G | 64.3% | 4.2 / 13.4 | 14 | intergenic (‑512/+91) | ompF/asnS | porin OmpF/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (9/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAA > NZ_CP009273/982820‑983073 | tGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAt > 1:443375/1‑138 (MQ=255) ccAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCGAATaa > 1:442328/1‑137 (MQ=255) tGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAAATTTCTTGATTTa > 2:193160/1‑138 (MQ=255) tgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGAt < 1:234434/139‑1 (MQ=255) tgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAAATTTCTTTACTTAAAAAACAGa > 2:172434/1‑138 (MQ=255) aaaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGaaaa < 1:172434/139‑1 (MQ=255) aTAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGt > 1:415870/1‑77 (MQ=255) aTAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGt < 2:415870/77‑1 (MQ=255) cGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAATAGATTGCTTTACTTAAAAAAAAAATAACTTGACAGAAAAGAAAAAAAAAAAACCAGCGGGCGCt > 1:576407/1‑139 (MQ=255) cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACg < 2:191501/139‑1 (MQ=255) cGTAACAGGATGATCGTTATGGCGGGGGTGCAAATAAAATGCTTTACTTTAAAAAAAAATAAATTGAAAGAAAAGAAAAAAAAAAAAGCAGCGGGCGCTGGCTCTTTAAGAAGCCAAAATAAAAAATGGCGGTAcacg > 1:411428/1‑138 (MQ=255) tAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAg < 1:193160/139‑1 (MQ=255) tAACAGGATGATCGTTATGGCGGGGGTGCACAAAGAATGCTTTACTTTACAAAAAAATAAAATTACAGAAAAGAAAAAAAAAAAGCCAGCGGGCGCCGGCGCTTTTAGAAGCTAAATTAGAAACAGGAGGTACGCGGAg > 2:204610/1‑139 (MQ=255) tAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATTTTTTACTTAAAAAAAAAATAAATTGACAGAAAAAAAAAAAAAAAAGACAACGGGGGCCGGGGCTTGTAGAAGCTAAATTAGAAAATGGGGTTACGCgggg > 2:542299/1‑137 (MQ=255) tgatCGTTATGGCGGGGGTTGAAAAAGATTGCTTGACTTAAAAAAAAGATAAATTTACAAAAAAGATAAAAAAAAAGACCACGGGGGCCGGCTCTTGAAGACGGTAAATTAGAAACTGGGGGTACGCGGGGGACGCggg > 1:290666/1‑139 (MQ=255) atCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGaa < 1:400896/139‑1 (MQ=255) | TGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAA > NZ_CP009273/982820‑983073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |