| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,363,786 | 0 | G | C | 58.3% | 3.6 / 14.7 | 12 | R46P (CGA→CCA) | pspD | phage shock protein PspD |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base C (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAG > NZ_CP009273/1363658‑1363902 | tCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGGGGTAAAAATACTTGCCTGCCCCCCGCTgga > 2:5215/1‑136 (MQ=255)tCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGGTAAAAACAGTTGTTCGCCCACCGCTGaa > 1:71138/1‑138 (MQ=255) ccGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCGGGGGGCTAAAATTAGGTGTTCGCCCACCGCCGGAAAAGTTgctgggcgt > 2:534734/1‑135 (MQ=255) ggTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGaa < 2:292414/139‑1 (MQ=255) tttCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGGTAAAAACAGGTGCTCCCCCACCGCTGGAAAAGTTGGTGGCTGTGGCGCTGGAACCGCTGGTTAGt > 1:296269/1‑139 (MQ=255) gCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGGCGGGGGGTGAAAACAGGTTGTCGCCCACCGCTGGAAATGGTGCTGGGGGGGGGGCTGGGAACGCTGTTTAAGCGGGGTGGtaata > 2:54571/1‑138 (MQ=255) gTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGca < 1:340904/138‑1 (MQ=255) cttACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGGCGGGGGGTAAAAACAACTGGTCTCCCGCCGCTGGCAAAGTTGGTTGCTGTGGGGCTTGGAAAGCCGTTTAGTCGGGGTGGCTATAAACAGGGCCAGccgt > 2:343519/1‑136 (MQ=255) tgGCGGGCTGGGCGGTAAAATCCGTTGCTCGCCCACCGCTGAAAATGTTGCTGGCTGTGGGGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGGGAGGGGAGTTTCGCCAAAAATg > 2:67663/1‑139 (MQ=255) ggCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAAtct < 1:307674/137‑1 (MQ=255) gCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAg < 1:214779/139‑1 (MQ=255) gCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAg < 1:54571/139‑1 (MQ=255) | TCGCTGGCAACAGGCCGGGCAAAAGGTAAAGCCTGGTTTCAAATTAGCAGGCAAGCTGGTACTTCTTACCGCACTGCGCTATGGCCCGGCGGGTGTGGCGGGCTGGGCGATAAAATCAGTTGCTCGCCGACCGCTGAAAATGTTGCTGGCTGTGGCGCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTTAAATCTCAG > NZ_CP009273/1363658‑1363902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |