| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,778,774 | 0 | C | T | 57.9% | 11.6 / 19.1 | 19 | A496V (GCT→GTT) | fadK | medium‑chain fatty‑acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (5/6); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAG > NZ_CP009273/1778642‑1778910 | ttttATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCtttttt < 1:56435/139‑1 (MQ=255) tGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCg > 1:471075/1‑139 (MQ=255) agcaTCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTa > 2:134349/1‑138 (MQ=255) aaaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGc < 2:364506/139‑1 (MQ=255) aaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCa < 2:512306/139‑1 (MQ=255) aaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCa < 2:111294/139‑1 (MQ=255) aTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaa < 1:41110/139‑1 (MQ=255) cACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCaaaa > 1:545646/1‑138 (MQ=255) cACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa > 1:213449/1‑138 (MQ=255) aTGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAaaatataa < 2:213312/139‑1 (MQ=255) tgtgGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCt < 2:471075/139‑1 (MQ=255) gTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTg > 1:443997/1‑139 (MQ=255) tCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTa > 1:161857/1‑139 (MQ=255) cgcTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTc < 2:161857/138‑1 (MQ=255) tGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATAc > 2:34753/1‑138 (MQ=255) tGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATAc > 2:5235/1‑138 (MQ=255) tGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCt < 2:439182/56‑1 (MQ=255) tGGAAGAGGTAGTGGTTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCt > 1:439182/1‑56 (MQ=255) ggCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAg > 1:129536/1‑139 (MQ=255) | TTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAG > NZ_CP009273/1778642‑1778910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |