Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,333,2750AG56.2% 7.4 / 16.6 16E77G (GAA→GGA) ubiGbifunctional 3‑demethylubiquinone 3‑O‑methyltransferase/2‑octaprenyl‑6‑hydroxy phenol methylase
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base G (9/0);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 4.81e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTCAAACCGCTGCACCGCATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGAGAGTATGGCGCGCGAAGGCGCGACGGTGACCGGTCTGGATATGGGCTTTGAGCCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGG  >  NZ_CP009273/2333143‑2333408
                                                                                                                                    |                                                                                                                                     
aGTTCAAACCGCTGCACCGCATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGGCTTTTGGCCGGGGGGATGGGGCGCGGAggggg                                                                                                                                 >  2:5048/1‑136 (MQ=255)
        ccGCTGCACCGCATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGAGAGTATGGCGCGCGAAGGCGCGACGGTGa                                                                                                                         <  1:304684/139‑1 (MQ=255)
            tGCACCGCATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGGATTCTGGCCGGGGGGATGGGGGGGGGAGGCGGGACGGGGAACgg                                                                                                                     >  1:124160/1‑139 (MQ=255)
                   cATTAACCCGCTGCGTCTGGGCTATATTGCCGGGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTTGGCGGGAGGATGGGGCGCGGAGGGGCGGCGGTGAACGGTCTGGa                                                                                                               >  1:503753/1‑138 (MQ=255)
                   cATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGGGAGGATGGGGCGCGGAGGGGCGGCGGGGACCGGGCTggg                                                                                                               >  1:438052/1‑137 (MQ=255)
                       aaCCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGAGAGTATGGCGCGCGAAGGCGCGACGGTGACCGGTCTGGATATg                                                                                                           <  2:52486/138‑1 (MQ=255)
                             cTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTTGGCGCGGGGATGGGGCGCGGGGGCGCGGCGGGGAGCGGTCTGGATATGGGGTTTg                                                                                                    >  2:301445/1‑139 (MQ=255)
                                                   aGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGAGAGTATGGCGCGCGGAGGCGCGGCGGGGACCGGGCTGGGTATGGGCTTTTAGCCATTGCAGGGGGCAAAACt                                                                              >  1:474663/1‑139 (MQ=255)
                                                     cGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGGCGAGAGGATTGGGCGCGGAGGCGCGACGGGGAACGGGCTGGATATGGGGTTTGTGCCCTTGGAGGTGGCAAAAATg                                                                             >  1:477866/1‑138 (MQ=255)
                                                        gCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTTTTGGCGGGAGGATGGGGCGCGGAAGGGCGGCGGGGACCGGGCTGGGTAAGGGCTTTGAGGCAATGGAGGGGGCAAAAATgcacg                                                                         >  2:388803/1‑139 (MQ=255)
                                                                                        tGTCGGTTGTGGCGGCGGCCTTCTGGCCGAGAGGATGGCGCGCGGAGGCGCGGCGGGGACCGGGCTGGGTATGGGGTTTGAGCCATTGCAGGGGGGCAAAATGCCCGCCCTGGGAAGCGGGCTTCCGGGGGGTTACggg                                         >  2:345535/1‑137 (MQ=255)
                                                                                                      gcggcATTCTGGCCGAGAGTATGGCGCGCGAAGGCGCGGCGGGGACCGGTCTGGGTATGGGCTTTGGGCCCTTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGGGGATTACGTGCAGGAAACCGTgg                            >  2:77786/1‑138 (MQ=255)
                                                                                                                           tGGCGCGCGAAGGCGCGACGGTGACCGGTCTGGATATGGGCTTTGAGCCATTGCAGGTGGCAAAACTGCATGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGc      >  1:293917/1‑139 (MQ=255)
                                                                                                                             gcgcgcGAAGGCGCGACGGTGACCGGTCTGGATA‑‑GGCTTTGAGCCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCgg   <  2:503753/138‑1 (MQ=255)
                                                                                                                               gcgcgAAGGCGCGACGGTGACCGGTCTGGATATGGGCTTTGAGCCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCggg  <  1:283788/139‑1 (MQ=255)
                                                                                                                               gcgcgAAGGCGCGACGGTGACCGGTCTGGATATGGGCTTTGAGCCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCggg  <  2:557698/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AGTTCAAACCGCTGCACCGCATTAACCCGCTGCGTCTGGGCTATATTGCCGAGCGTGCTGGCGGTTTATTTGGCAAAAAGGTGCTCGATGTCGGTTGTGGCGGCGGCATTCTGGCCGAGAGTATGGCGCGCGAAGGCGCGACGGTGACCGGTCTGGATATGGGCTTTGAGCCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGG  >  NZ_CP009273/2333143‑2333408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑