Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,634,3410CT56.7% ‑2.6 / 27.2 30F40F (TTC→TTT) dtpBdipeptide/tripeptide permease DtpB
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base T (17/0);  total (22/8)
Fisher's exact test for biased strand distribution p-value = 2.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCT  >  NZ_CP009273/3634205‑3634478
                                                                                                                                        |                                                                                                                                           
ttGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttct                                                                                                                                            <  2:162711/138‑1 (MQ=255)
 tGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttcttc                                                                                                                                          >  1:184699/1‑139 (MQ=255)
       gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCCACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGTGCGTACTGGCGGGTTTTTTCTTT‑‑aaa                                                                                                                                  >  2:109902/1‑139 (MQ=255)
       gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑aaa                                                                                                                                  <  2:36032/139‑1 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑taa                                                                                                                                  >  2:153009/1‑138 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑taa                                                                                                                                  >  1:74636/1‑138 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑aaa                                                                                                                                  >  1:360970/1‑138 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCg                                                                                                                                         <  2:474920/133‑1 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCg                                                                                                                                         >  1:474920/1‑133 (MQ=255)
        ttAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGCTTTTTTTTTT‑‑aaa                                                                                                                                  >  1:157198/1‑138 (MQ=255)
                 atGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑AAAAAGCTTgggt                                                                                                                        >  1:112140/1‑137 (MQ=255)
                  tGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttct                                                                                                                                            <  2:365526/120‑1 (MQ=255)
                  tGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttct                                                                                                                                            >  1:365526/1‑120 (MQ=255)
                      tACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGATTTTTTTCTT‑‑TTACACCCTGGATTTTCg                                                                                                                   >  2:333659/1‑139 (MQ=255)
                        caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGc                                                                                                                  <  1:109902/138‑1 (MQ=255)
                           caacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑TAAAAGCCTTGCTTTTCGCaa                                                                                                                >  2:557753/1‑137 (MQ=255)
                           caacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTCTT‑‑TAAAAACTTTGATTTTCGcca                                                                                                                >  2:544806/1‑135 (MQ=255)
                                   aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑AAAAAGCTTGGGTTTTCGCAAGAGCAGGcct                                                                                                      >  2:423976/1‑137 (MQ=255)
                                   aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTCGTT‑‑TAAAAGCTTTGGTTCTCGCAAGAGCAGGCtt                                                                                                      >  1:554482/1‑139 (MQ=255)
                                          tgctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTT‑‑TAACAGCTTGGGTTTTCGCAAGAGGAGGCTTTTTTCAc                                                                                               >  2:131092/1‑139 (MQ=255)
                                             tgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTT‑‑AAAAAGCCTTGATTTTCGCCAAAGGCGGCGTTTTTCACttt                                                                                            >  1:349160/1‑139 (MQ=255)
                                               cagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑AAACAGCTTTGGTTCTCGCAAGAGCAGGCTTTTGTTACttttt                                                                                          >  2:274340/1‑138 (MQ=255)
                                               cagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACttt                                                                                            <  2:348142/137‑1 (MQ=255)
                                               cagcaACCTCGCCCATTTTTAATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTTTTTCAAACA‑CTTGGGTT‑TCGCAAGAGGCGGCGTTTGTTACttttt                                                                                          >  1:200265/1‑138 (MQ=255)
                                                 gcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTT‑‑TAACAGCTTGGGTTTTCGCAAGAGCAGGGTTTTTTTTCTTTTTGt                                                                                        >  2:444217/1‑139 (MQ=255)
                                                 gcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑TAAAAGCTTTGGTTCTCGCAAGAGCAGGGTTTTGTCACTTTTTGt                                                                                        >  1:441681/1‑139 (MQ=255)
                                                         gCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTtg                                                                                 <  2:184699/138‑1 (MQ=255)
                                                              tttttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTT‑‑TAACAGCCTGGGTTCTCGCCAGAGCAGGGTTTTTTCACTTTTTGTGGTTTTTCTgcgc                                                                           >  1:196359/1‑139 (MQ=255)
                                                                       aTCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTAt                                                                   <  1:494026/138‑1 (MQ=255)
                                                                                              gCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTT‑‑TAAAAGCTTGGGTTTTCGCAAGAGCAGGCGTTTTTCACTTTTGGTGTTTTTTCTGCGCCGGGCTATGGCCTCCTTTTCATTtgcgggtgt                                           >  2:110396/1‑135 (MQ=255)
                                                                                                gATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttct                                                                                                                                            <  2:465701/42‑1 (MQ=255)
                                                                                                          ctacGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGaccac                               <  2:349160/139‑1 (MQ=255)
                                                                                                                       ggCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTTTCACTTTTTGTGCTTTTTCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATTTCGGCGACCACCCGCTGGGGGcc                   >  1:395695/1‑138 (MQ=255)
                                                                                                                            aCTGGCGGTTTTTTTCGTT‑‑AAACAGCTTGGGTTCTCGCAAGAGCAGGCTTTTTTCACTTTTTGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTTCATTGGGGGGTATTTTTGGGGCCACCCGCTTGGGGCCaaaac              >  1:466115/1‑136 (MQ=255)
                                                                                                                                  ggTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCAtt       <  1:444217/139‑1 (MQ=255)
                                                                                                                                        cttcGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCt  <  2:200265/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTT‑‑AAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCT  >  NZ_CP009273/3634205‑3634478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑