Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,872,9510TC61.9% 21.0 / 50.0 42T177A (ACC→GCC) gyrBDNA topoisomerase (ATP‑hydrolyzing) subunit B
Reads supporting (aligned to +/- strand):  ref base T (7/9);  new base C (11/15);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCGCCTTCATAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCT  >  NZ_CP009273/3872816‑3873052
                                                                                                                                       |                                                                                                     
tGCCGCCTTCATAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGaa                                                                                                    <  1:337250/139‑1 (MQ=255)
   cgccTTCATAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGt                                                                                                 >  2:343385/1‑139 (MQ=255)
         cATAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAg                                                                                           >  1:576290/1‑139 (MQ=255)
           tAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGc                                                                                         <  2:62356/139‑1 (MQ=255)
           tAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGc                                                                                         <  2:412835/139‑1 (MQ=255)
           tAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGc                                                                                         <  1:31867/139‑1 (MQ=255)
                gAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGc                                                                                    <  1:156951/139‑1 (MQ=255)
                   gTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGc                                                                                    >  1:481275/1‑136 (MQ=255)
                      gTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTc                                                                                              >  1:368055/1‑123 (MQ=255)
                      gTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTc                                                                                              <  2:368055/123‑1 (MQ=255)
                      gTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGaaa                                                                              >  2:135795/1‑139 (MQ=255)
                       tcttctTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAAc                                                                             >  2:67809/1‑139 (MQ=255)
                                ccGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATgg                                                                    >  2:180159/1‑139 (MQ=255)
                                  gTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTg                                                                  >  2:13295/1‑139 (MQ=255)
                                       gcTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGt                                                             <  1:13295/139‑1 (MQ=255)
                                                 cAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGtc                                                   <  1:271546/139‑1 (MQ=255)
                                                            aaaCGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAAc                                        >  1:285230/1‑139 (MQ=255)
                                                                      gTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCggg                              <  1:348878/139‑1 (MQ=255)
                                                                      gTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCggg                              <  1:565545/139‑1 (MQ=255)
                                                                         gAGGAACGCCAACGCTGGCAGTCGTTCCGCCAGAATTTCATATTCGAATGCGGTCCCATGGTTGAAGGTTTCGCGGCTGGACCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGc                            <  2:60664/138‑1 (MQ=255)
                                                                         gAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGc                            <  1:343385/138‑1 (MQ=255)
                                                                         gAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGc                            <  2:182727/138‑1 (MQ=255)
                                                                         gAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTGCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGc                            <  2:75701/138‑1 (MQ=255)
                                                                         aagaaaCGACACCCCACGCAGACGTTTCGCCAGAATTGCATATTCGAACCCGGTCCCTTTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCCCCAGGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGc                            <  2:357337/134‑1 (MQ=255)
                                                                          aGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCGGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCggg                              <  1:139541/135‑1 (MQ=255)
                                                                          aGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCggg                              >  2:139541/1‑135 (MQ=255)
                                                                          aGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCt                          >  1:306262/1‑139 (MQ=255)
                                                                          aGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCt                          >  1:276393/1‑139 (MQ=255)
                                                                               cGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCgg                      >  2:216029/1‑138 (MQ=255)
                                                                                gACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTa                    <  2:481275/139‑1 (MQ=255)
                                                                                 aCAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGaa                                                                               >  1:67482/1‑79 (MQ=255)
                                                                                 aCAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGaa                                                                               <  2:67482/79‑1 (MQ=255)
                                                                                  cAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTaca                  <  1:67809/139‑1 (MQ=255)
                                                                                    ccacACGCGGACGTTTCGCCAGAATTTCATATTCGAACGCGATCCCATTGGTGAAGGTTTCGAGGCTGGGCCAGAACCGCACCATGGTGCCGTTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACAcc                <  2:55297/136‑1 (MQ=255)
                                                                                        aCGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCgt              <  2:354109/137‑1 (MQ=255)
                                                                                               cGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCgt              <  2:191227/130‑1 (MQ=255)
                                                                                               cGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGCGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCgt              >  1:191227/1‑130 (MQ=255)
                                                                                                  ttCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCt  >  1:302722/1‑139 (MQ=255)
                                                                                                               cATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGtttttt                                                        >  1:278588/1‑72 (MQ=255)
                                                                                                               cATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGtttttt                                                        <  2:278588/72‑1 (MQ=255)
                                                                                                                                      gTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAAc                                        >  1:248400/1‑65 (MQ=255)
                                                                                                                                      gTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAAc                                        <  2:248400/65‑1 (MQ=255)
                                                                                                                                       |                                                                                                     
TGCCGCCTTCATAGTGGAAGTGGTCTTCTTTGCCGTCGCGCTTGTCGCGCAGACGAATGGAAACGCCGGAGTTGAGGAACGACAACTCACGCAGACGTTTCGCCAGAATTTCATATTCGAACTCGGTCACATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCT  >  NZ_CP009273/3872816‑3873052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑