Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,228,3260TC71.0% 35.1 / 24.0 31V255V (GTT→GTC) yjbHYjbH domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base C (9/13);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTG  >  NZ_CP009273/4228192‑4228458
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aCGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAAcc                                                                                                                                  <  2:274230/139‑1 (MQ=255)
aCGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAAcc                                                                                                                                  <  1:391154/139‑1 (MQ=255)
 cGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCt                                                                                                                                 <  2:531952/139‑1 (MQ=255)
    ccTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAg                                                                                                                              >  1:269602/1‑139 (MQ=255)
                tGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTgg                                                                                                                  >  2:74856/1‑139 (MQ=255)
                 gcgcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGCGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTCTGAACGTgg                                                                                                                  >  2:412816/1‑138 (MQ=255)
                   gcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCaa                                                                                                               <  2:525277/139‑1 (MQ=255)
                   gcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCaa                                                                                                               <  1:432062/139‑1 (MQ=255)
                   gcCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCaa                                                                                                               <  2:184146/139‑1 (MQ=255)
                              gAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGt                                                                                                    >  1:110160/1‑139 (MQ=255)
                               aGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGtt                                                                                                   <  2:436452/139‑1 (MQ=255)
                               aGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGtt                                                                                                   <  2:460272/139‑1 (MQ=255)
                                   tGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGtt                                                                                                                                      <  1:110686/100‑1 (MQ=255)
                                   tGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGtt                                                                                                                                      >  2:110686/1‑100 (MQ=255)
                                        gCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTAc                                                                                          >  2:473558/1‑139 (MQ=255)
                                                      cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCa                                                                                                                <  2:496696/103‑1 (MQ=255)
                                                      cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCa                                                                                                                >  1:496696/1‑103 (MQ=255)
                                                      cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCaa                                                                                                               <  1:323825/104‑1 (MQ=255)
                                                      cagGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCaa                                                                                                               >  2:323825/1‑104 (MQ=255)
                                                          aTTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        <  1:102180/139‑1 (MQ=255)
                                                          aTTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        <  1:74856/139‑1 (MQ=255)
                                                          aTTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        <  2:532716/139‑1 (MQ=255)
                                                          aTTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        <  1:473558/139‑1 (MQ=255)
                                                                     aaGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGcc                                                             <  2:110160/139‑1 (MQ=255)
                                                                                                   ggTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        >  2:296264/1‑98 (MQ=255)
                                                                                                   ggTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTaa                                                                        <  1:296264/98‑1 (MQ=255)
                                                                                                    gTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCccgcccgc                                  <  2:33024/135‑1 (MQ=255)
                                                                                                    gTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCccgcccgc                                  >  1:33024/1‑135 (MQ=255)
                                                                                                    gTGCGATTTATCGCGTTACCGATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACgccggccccaaatt                              >  1:443819/1‑133 (MQ=255)
                                                                                                                   ttACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAAccgcagccgca               <  1:106547/139‑1 (MQ=255)
                                                                                                                        gATTGGGCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATg          >  1:134981/1‑139 (MQ=255)
                                                                                                                            gggCCGACGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCa       <  2:563456/138‑1 (MQ=255)
                                                                                                                                  aCGTCAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTg  >  1:443200/1‑137 (MQ=255)
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ACGCCCTGGCAACCGCTGCGCCTGAAACTGGAGTATGAAGGCAATAATTATCAGCAGGATTTTGCCGGGAAGCTGGAGCAAAAAAGTAAGTTTAACGTCGGTGCGATTTATCGCGTTACCGATTGGGCCGACGTTAACCTTAGCTATGAACGTGGCAACACCTTTATGTTTGGCGTTACGTTGCGCACCAACTTTAACGATCTGCGCCCGTCTTACAACGATAACGCCCGCCCGCAATATCAACCGCAGCCGCAGGATGCCATTTTG  >  NZ_CP009273/4228192‑4228458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑