Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	225,426	T→C	noncoding (12/77 nt)	BW25113_RS01035 →	tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	225,426	0	T	C	100.0%	24.3 / NA	8	noncoding (12/77 nt)	BW25113_RS01035	tRNA‑Asp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/7);  total (1/7)

CCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGCC  >  NZ_CP009273/225391‑225553                                    |                                                                                                                                ccGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGtttttt                          <  1:104057/139‑1 (MQ=255)     tggtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCg                      >  2:349623/1‑139 (MQ=255)       gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGt                     <  2:130466/138‑1 (MQ=255)       gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTc                    <  1:65961/139‑1 (MQ=255)           tAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGt                 <  1:532672/138‑1 (MQ=255)              aaGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTatat             <  1:437650/139‑1 (MQ=255)                   ttCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCtatt        <  1:530831/139‑1 (MQ=255)                          gAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGcc  <  1:85721/138‑1 (MQ=255)                                    |                                                                                                                                CCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGCC  >  NZ_CP009273/225391‑225553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑