Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,449,980	A→G	D187G (GAT→GGT)	paaC →	phenylacetate‑CoA oxygenase subunit PaaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,449,980	0	A	G	100.0%	60.3 / NA	18	D187G (GAT→GGT)	paaC	phenylacetate‑CoA oxygenase subunit PaaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

ACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCAT  >  NZ_CP009273/1449849‑1450115                                                                                                                                    |                                                                                                                                        aCTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGc                                                                                                                                  <  1:345669/139‑1 (MQ=255)       cAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTa                                                                                                                            <  1:508023/139‑1 (MQ=255)                       tCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAg                                                                                                            >  2:388805/1‑139 (MQ=255)                       tCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAg                                                                                                            >  2:193150/1‑139 (MQ=255)                            gCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCg                                                                                                         >  2:412314/1‑137 (MQ=255)                                               aTCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACg                                                                                    <  2:539318/139‑1 (MQ=255)                                                                                ttCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCg                                                    <  1:193150/138‑1 (MQ=255)                                                                                     tGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCg                                               >  2:401493/1‑138 (MQ=255)                                                                                          acgaGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTgg                                         >  1:404881/1‑139 (MQ=255)                                                                                                             gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGa                            <  2:61839/133‑1 (MQ=255)                                                                                                             gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGa                            >  1:61839/1‑133 (MQ=255)                                                                                                                   agagGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa                <  1:392040/139‑1 (MQ=255)                                                                                                                   agagGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa                <  1:322528/139‑1 (MQ=255)                                                                                                                        gTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCt           >  2:152095/1‑139 (MQ=255)                                                                                                                        gTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCt           >  2:220971/1‑139 (MQ=255)                                                                                                                        gTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCt           >  1:85985/1‑139 (MQ=255)                                                                                                                        gTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGGAAAAAAGGACTGCATACCGAACACCt           >  2:59265/1‑139 (MQ=255)                                                                                                                                 ttGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCAt  >  1:106808/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        ACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCAT  >  NZ_CP009273/1449849‑1450115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑