Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,492,661	A→G	M517V (ATG→GTG)	opgD →	glucan biosynthesis protein OpgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,492,661	0	A	G	100.0%	115.5 / NA	33	M517V (ATG→GTG)	opgD	glucan biosynthesis protein OpgD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

TTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGG  >  NZ_CP009273/1492527‑1492796                                                                                                                                       |                                                                                                                                        ttGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCAATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTa                                                                                                                                     <  1:16540/139‑1 (MQ=255)                cGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAgg                                                                                                                       <  1:96306/138‑1 (MQ=255)                           ggggAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTAt                                                                                                             >  2:189825/1‑137 (MQ=255)                               aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTAt                                                                                                             >  2:570980/1‑133 (MQ=255)                               aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTAt                                                                                                             <  1:570980/133‑1 (MQ=255)                               aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTg                                                                                                        >  1:453008/1‑138 (MQ=255)                                  cgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAAc                                                                                                    <  2:158446/139‑1 (MQ=255)                                    aagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACAt                                                                                                  <  1:179171/139‑1 (MQ=255)                                      gcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATgg                                                                                                <  1:354245/139‑1 (MQ=255)                                               aaTTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAg                                                                                       <  1:395584/139‑1 (MQ=255)                                                           tGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGc                                                                             >  2:528783/1‑137 (MQ=255)                                                               cccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcgc                                                                       >  1:470103/1‑139 (MQ=255)                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACg                                                                                                                               <  1:172677/80‑1 (MQ=255)                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACg                                                                                                                               >  2:172677/1‑80 (MQ=255)                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAg                                                                                       <  1:46518/120‑1 (MQ=255)                                                                  aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAg                                                                                       >  2:46518/1‑120 (MQ=255)                                                                         gTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACg                                                             >  1:281714/1‑139 (MQ=255)                                                                          ttATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                                            <  2:453008/139‑1 (MQ=255)                                                                             tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCa                                                          <  1:424350/138‑1 (MQ=255)                                                                               gTATTCAGTTTGACTGGTATCCGACTTCGGATTCCACTGATCCGTTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                        <  2:503272/138‑1 (MQ=255)                                                                               gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                        <  1:189825/138‑1 (MQ=255)                                                                                          gACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATgg                                                                                                >  1:172955/1‑87 (MQ=255)                                                                                          gACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATgg                                                                                                <  2:172955/87‑1 (MQ=255)                                                                                           aCTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGAcc                                            <  2:328816/138‑1 (MQ=255)                                                                                               gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtg                                       <  1:528783/139‑1 (MQ=255)                                                                                                          cGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTc                                                           <  1:560535/108‑1 (MQ=255)                                                                                                          cGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTc                                                           >  2:560535/1‑108 (MQ=255)                                                                                                          cGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATc                            >  1:221241/1‑139 (MQ=255)                                                                                                                           gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCAcc           >  1:204526/1‑139 (MQ=255)                                                                                                                           gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCAcc           >  2:216515/1‑139 (MQ=255)                                                                                                                           gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCAcc           >  1:235875/1‑139 (MQ=255)                                                                                                                                tatGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACcttctt      >  1:394633/1‑139 (MQ=255)                                                                                                                                    cGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCggg  <  2:235875/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGG  >  NZ_CP009273/1492527‑1492796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑