| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,506,028 | G→A | A40T (GCC→ACC) | ydcS → | ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,506,028 | 0 | G | A | 92.9% | 45.2 / NA | 14 | A40T (GCC→ACC) | ydcS | ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (8/5); minor base C (0/1); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGTCTG > NZ_CP009273/1505911‑1506156 | attaGCAACACATTTGCCCGCAGCAGCCTGTGTCCGCTCAGCATGGCAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGag < 2:135630/135‑2 (MQ=255) cAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGa > 1:6089/1‑139 (MQ=255) aGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGAc > 1:414485/1‑138 (MQ=255) aGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa < 1:435797/139‑1 (MQ=255) aGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACa < 2:428755/139‑1 (MQ=255) ttGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGCCTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGa > 2:84215/1‑139 (MQ=255) cGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATaaa > 2:158990/1‑138 (MQ=255) aTGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCg < 1:81552/139‑1 (MQ=255) cGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGc > 2:34961/1‑139 (MQ=255) ccTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGaa > 1:562635/1‑63 (MQ=255) ccTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGaa < 2:562635/63‑1 (MQ=255) tAGATAAACCGGAAGGGCGACTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGAc > 1:327807/1‑139 (MQ=255) aCTGGATATTATCACCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGt > 2:343210/1‑139 (MQ=255) gATATTATCCCCTGGCCGGAATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGAAACGCAGTTCAAAAAAGAGAAAGGCGGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGTCtg < 2:520114/138‑1 (MQ=255) | ATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACGCAGTTCGAAAAAGAGACAGGCTGCGCGGTGAATGTGAAAACCGCCGCGACTTCCGATGAAATGGTCAGTCTG > NZ_CP009273/1505911‑1506156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |