Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,542,720	A→G	T355A (ACT→GCT)	fdnG →	formate dehydrogenase‑N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,542,720	0	A	G	100.0%	99.8 / NA	27	T355A (ACT→GCT)	fdnG	formate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/17);  total (10/17)

CCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCAC  >  NZ_CP009273/1542584‑1542847                                                                                                                                         |                                                                                                                                ccTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCt                                                                                                                               <  2:389186/139‑1 (MQ=255)     ctgGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCAt                                                                                                                            <  1:254109/138‑1 (MQ=255)         tGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgcg                                                                                                                       <  1:147397/139‑1 (MQ=255)         tGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCcgcg                                                                                                                       <  2:560846/139‑1 (MQ=255)           cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCgcgc                                                                                                                      <  2:529138/138‑1 (MQ=255)             tgatgaTTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCtg                                                                                                                    >  2:26592/1‑138 (MQ=255)             tgatgaTTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCtg                                                                                                                    <  1:26592/138‑1 (MQ=255)                        cTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAAcc                                                                                                         <  1:367145/138‑1 (MQ=255)                                                 cTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTa                                                                                >  2:396892/1‑138 (MQ=255)                                                 cTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTa                                                                                >  1:209076/1‑138 (MQ=255)                                                   acgacgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTacac                                                                             >  1:104248/1‑139 (MQ=255)                                                   acgacgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTacac                                                                             >  1:527942/1‑139 (MQ=255)                                                                            tAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGc                                                    >  2:237345/1‑139 (MQ=255)                                                                               aTCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGt                                                 <  2:226304/139‑1 (MQ=255)                                                                                    ccTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGc                                             >  1:16756/1‑138 (MQ=255)                                                                                              tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGa                                   <  1:396892/138‑1 (MQ=255)                                                                                              tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGa                                   <  2:104248/138‑1 (MQ=255)                                                                                              tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGa                                   <  2:527942/138‑1 (MQ=255)                                                                                              tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGAc                                  >  1:416101/1‑139 (MQ=255)                                                                                                aGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACt                                 <  2:416101/138‑1 (MQ=255)                                                                                                      aTGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGaa                          <  2:16756/139‑1 (MQ=255)                                                                                                      aTGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGa                           >  1:381599/1‑138 (MQ=255)                                                                                                       tGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGaaa                         <  2:209076/139‑1 (MQ=255)                                                                                                              ggCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGtgtgtg                  <  1:237345/139‑1 (MQ=255)                                                                                                                   tGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTg             >  1:120768/1‑139 (MQ=255)                                                                                                                      gAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGGGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTg          <  1:92343/139‑1 (MQ=255)                                                                                                                              aTGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTccac  <  2:177527/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                CCTGCTGGTGCGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCAC  >  NZ_CP009273/1542584‑1542847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑