Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,580,214	T→C	D177G (GAC→GGC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,580,214	0	T	C	100.0%	72.0 / NA	22	D177G (GAC→GGC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

TTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTATAGCTTTGAAGG  >  NZ_CP009273/1580079‑1580333                                                                                                                                        |                                                                                                                        tttGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCgg                                                                                                                      <  2:370265/139‑1 (MQ=255)            gttactcccAATGCAGATGACTAAATCGCCCATCTCAAAGTCTTCCAGCAACACGGTCCCTTTCCCTACCCCGATACTCCCTGCGAAACCCACGCATGTCGGTTCATGGCTCATGTTGGAGCAGCCGGGAAAGGTATTg                                                                                                           <  2:508701/133‑1 (MQ=255)            gTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTg                                                                                                           <  1:485590/139‑1 (MQ=255)                      aTGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTAtt                                                                                                 >  2:358266/1‑139 (MQ=255)                                   aaTCGCACTTCTCAAAGTCCTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAg                                                                                    >  1:104893/1‑139 (MQ=255)                                        cACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCt                                                                                                         >  2:546759/1‑113 (MQ=255)                                        cACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCt                                                                                                         <  1:546759/113‑1 (MQ=255)                                                        ccAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCtt                                                                <  2:112150/138‑1 (MQ=255)                                                                      ccTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                                 <  2:548515/139‑1 (MQ=255)                                                                                     aTACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt                                  >  1:487342/1‑139 (MQ=255)                                                                                     aTACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt                                  >  2:301952/1‑139 (MQ=255)                                                                                      tACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt                                  >  1:401718/1‑138 (MQ=255)                                                                                                  aaCCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTggg                     <  2:104893/139‑1 (MQ=255)                                                                                                       aCGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                              <  1:18034/109‑1 (MQ=255)                                                                                                       aCGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                              >  2:18034/1‑109 (MQ=255)                                                                                                       aCGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCAc                >  1:335522/1‑139 (MQ=255)                                                                                                       aCGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCAc                >  1:281313/1‑139 (MQ=255)                                                                                                         gCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACta              <  2:552471/139‑1 (MQ=255)                                                                                                              tCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAAc                              >  1:78830/1‑118 (MQ=255)                                                                                                              tCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAAc                              <  2:78830/118‑1 (MQ=255)                                                                                                                     aTGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTATAGCTTTGAAgg  >  2:205486/1‑139 (MQ=255)                                                                                                                     aTGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTATAGCTTTGAAgg  >  2:510586/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                        TTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTATAGCTTTGAAGG  >  NZ_CP009273/1580079‑1580333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑