| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,599,483 | A→C | D59A (GAT→GCT) | lsrB → | autoinducer 2 ABC transporter substrate‑binding protein LsrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,599,483 | 0 | A | C | 100.0% | 87.5 / NA | 25 | D59A (GAT→GCT) | lsrB | autoinducer 2 ABC transporter substrate‑binding protein LsrB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (14/11); total (14/11) | |||||||||||
GCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACG > NZ_CP009273/1599347‑1599618 | gcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCtg < 2:501164/139‑1 (MQ=255) ttGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCtg > 2:463758/1‑135 (MQ=255) ttGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCtg < 1:463758/135‑1 (MQ=255) gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTacgacg > 2:307773/1‑139 (MQ=255) tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCATCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAgtgt > 1:322036/1‑139 (MQ=255) tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAgtgt > 1:385821/1‑139 (MQ=255) cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGttt < 2:176778/139‑1 (MQ=255) ccGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTgg < 1:470004/139‑1 (MQ=255) gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTc < 1:159358/139‑1 (MQ=255) tATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAg > 1:304261/1‑139 (MQ=255) ttGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGtt < 1:307773/139‑1 (MQ=255) ggTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGtcaat < 2:385821/139‑1 (MQ=255) gTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGtcaat > 2:572594/1‑138 (MQ=255) gATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATaa > 1:383576/1‑139 (MQ=255) gCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGt > 1:427706/1‑139 (MQ=255) gTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTc > 2:550285/1‑139 (MQ=255) cgcACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGATCCCa < 1:83647/62‑1 (MQ=255) cgcACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCa > 2:83647/1‑62 (MQ=255) gCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCt > 1:475328/1‑139 (MQ=255) cGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCtt < 1:303141/139‑1 (MQ=255) ggTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTtgt > 2:115670/1‑139 (MQ=255) aaGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTcc > 2:456515/1‑139 (MQ=255) aaGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTc < 1:550285/138‑1 (MQ=255) gggCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACt > 1:270013/1‑138 (MQ=255) ttGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAAcg < 2:304261/139‑1 (MQ=255) | GCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACG > NZ_CP009273/1599347‑1599618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |