| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,608,701 | C→A | R87L (CGC→CTC) | sad ← | succinate‑semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,608,701 | 0 | C | A | 100.0% | 23.7 / NA | 7 | R87L (CGC→CTC) | sad | succinate‑semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (4/3); total (4/3) | |||||||||||
AGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGCACGAT > NZ_CP009273/1608569‑1608806 | aGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCt > 2:274726/1‑139 (MQ=255) ctgctgATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCAtt < 2:70590/139‑1 (MQ=255) ggTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGa < 2:27519/138‑1 (MQ=255) tACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAg < 2:578420/139‑1 (MQ=255) aCCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAg > 2:334927/1‑138 (MQ=255) tCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGCNc > 1:125175/1‑136 (MQ=255) tCACACAAATTCGCCGATTTCGCCACTTCAGCGAGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATAT‑ACGCAGTTTTTCAGCACGAt > 2:544441/1‑138 (MQ=255) | AGAATCGTCCCCAACGGTCGATACTCAATAACCGCCTGCTGATTTTCCACCAGCGTAGGTTCCGCCTTCAGCATTGCCGGACCATGTTCTGCATACCAGTCACACAAATTCGCCGATTTCGCCACTTCAGCGCGCGCCTGGTTGATTGGTTTGCCCATTTCGCGGGTGATCATTTGCGCCATTTCTTCGCTACGAGCGCGCAGAGCCTTACCGATATCACGCAGTTTTTCAGCACGAT > NZ_CP009273/1608569‑1608806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |