Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,622,039	A→G	E89G (GAG→GGG)	ydfG →	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,622,039	0	A	G	100.0%	59.2 / NA	18	E89G (GAG→GGG)	ydfG	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

GGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCAT  >  NZ_CP009273/1621906‑1622157                                                                                                                                      |                                                                                                                       ggAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGcc                                                                                                                     <  1:19105/137‑1 (MQ=255)      tAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCAt                                                                                                              <  2:515784/139‑1 (MQ=255)                    cccAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                                                 >  2:455914/1‑138 (MQ=255)                              cGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGa                                                                                       >  1:188658/1‑138 (MQ=255)                                 tCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAAc                                                                                    >  1:277342/1‑138 (MQ=255)                                                         gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                           <  1:555352/139‑1 (MQ=255)                                                         gaTGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                           <  2:188658/139‑1 (MQ=255)                                                                      cTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                           >  1:116903/1‑126 (MQ=255)                                                                      cTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGc                                                           <  2:116903/126‑1 (MQ=255)                                                                          cTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGAcgcg                                          <  1:455914/139‑1 (MQ=255)                                                                                               atatCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGt                     >  1:5721/1‑139 (MQ=255)                                                                                                    cTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaacaa                                                               <  2:184541/92‑1 (MQ=255)                                                                                                    cTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaacaa                                                               >  1:184541/1‑92 (MQ=255)                                                                                                       gTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGt                                    >  1:333860/1‑116 (MQ=255)                                                                                                       gTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGt                                    <  2:333860/116‑1 (MQ=255)                                                                                                                 ccGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTa                         <  1:455606/117‑1 (MQ=255)                                                                                                                 ccGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTa                         >  2:455606/1‑117 (MQ=255)                                                                                                                  cGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCat  <  1:299859/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                       GGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCAT  >  NZ_CP009273/1621906‑1622157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑