Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	238,914	A→C	F293V (TTT→GTT)	fadE ←	acyl‑CoA dehydrogenase FadE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	238,914	0	A	C	100.0%	114.3 / NA	32	F293V (TTT→GTT)	fadE	acyl‑CoA dehydrogenase FadE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/14);  total (18/14)

GGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCG  >  NZ_CP009273/238790‑239026                                                                                                                             |                                                                                                                 ggTACGTTGGGCGGGAGGTGGCGACGACCATTTCCCCCGCCCGGCGTGGTGGTTGGGATCAGCCCACAGGTAATGCCAAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCa                                                                                                    <  2:47075/139‑1 (MQ=255)  gTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCAc                                                                                                   >  1:491820/1‑139 (MQ=255)  gTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCAc                                                                                                   >  1:47075/1‑139 (MQ=255)           gCGGAAAGGGGCGACGACAAATTTCCACGCCCGGCGGGGTGGTTGGGATCGGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAAt                                                                                          <  1:362723/139‑1 (MQ=255)            cGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACTGTctctta                                                                                          >  1:471963/1‑133 (MQ=255)            cGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATc                                                                                         >  2:343128/1‑139 (MQ=255)                 aGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGc                                                                                    >  2:293940/1‑139 (MQ=255)                                            gCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTGGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGt                                                         <  1:343128/139‑1 (MQ=255)                                                ggtggtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCCCCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGa                                                     <  1:96275/139‑1 (MQ=255)                                                    gtTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCTGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGc                                                  <  1:484941/138‑1 (MQ=255)                                                       gggATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATcc                                              >  2:93217/1‑139 (MQ=255)                                                            cAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAg                                          >  1:350513/1‑138 (MQ=255)                                                               cgcACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCAcc                                      >  1:24665/1‑139 (MQ=255)                                                                  acaGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACctgc                                   >  1:46677/1‑139 (MQ=255)                                                                       tAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGcc                              >  1:276991/1‑139 (MQ=255)                                                                       tAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTCCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGcc                              <  1:580926/139‑1 (MQ=255)                                                                         aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGc                                                                                                               >  2:91525/1‑56 (MQ=255)                                                                         aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGc                                                                                                               <  1:91525/56‑1 (MQ=255)                                                                         aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGccc                             <  1:342007/138‑1 (MQ=255)                                                                         aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCt                            >  1:62170/1‑139 (MQ=255)                                                                         aTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCt                            >  1:165280/1‑139 (MQ=255)                                                                              tAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCa                                                       >  2:29364/1‑107 (MQ=255)                                                                              tAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCa                                                       <  1:29364/107‑1 (MQ=255)                                                                                aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGATTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCAtt                      <  2:238033/138‑1 (MQ=255)                                                                                aaTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCAtt                      >  1:238033/1‑138 (MQ=255)                                                                                      ctGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCAt               >  2:39339/1‑139 (MQ=255)                                                                                      cggccccgccgAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCAt               <  2:170245/134‑1 (MQ=255)                                                                                           ccgccgAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGa          <  2:62170/139‑1 (MQ=255)                                                                                            cgccgAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGa          >  2:282382/1‑138 (MQ=255)                                                                                              ccgAGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACaa       <  1:93217/139‑1 (MQ=255)                                                                                                 aGTAATTTTTCCGGGTCGGAGAGTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATcc    >  1:559176/1‑139 (MQ=255)                                                                                                   tAATTTTTCCGGGTCGGAGATTTTAACCGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCg  <  2:113632/139‑1 (MQ=255)                                                                                                                             |                                                                                                                 GGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCATCCCCAGCACCTGCTGGCCCTGCCATTCGCCCATGCAGACAATCCCG  >  NZ_CP009273/238790‑239026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑