Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,707,599	A→T	I192F (ATC→TTC)	dtpA →	dipeptide/tripeptide permease DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,707,599	0	A	T	100.0%	123.0 / NA	31	I192F (ATC→TTC)	dtpA	dipeptide/tripeptide permease DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/13);  total (18/13)

TGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCT  >  NZ_CP009273/1707475‑1707734                                                                                                                             |                                                                                                                                        tGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      >  2:582607/1‑128 (MQ=255) tGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      <  1:582607/128‑1 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      <  2:489286/118‑1 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCa                                                                                                                                      >  1:489286/1‑118 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCt                                                                                                                 >  1:330198/1‑139 (MQ=255)           aTGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCt                                                                                                                 >  1:545527/1‑139 (MQ=255)               actacATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGcc                                                                                                              >  2:478480/1‑138 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  1:259893/138‑1 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  2:545527/138‑1 (MQ=255)                   cATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  2:375290/138‑1 (MQ=255)                    aTGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCt                                                                                                        <  1:402268/139‑1 (MQ=255)                      gTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTgg                                                                                                      >  2:27422/1‑139 (MQ=255)                                aTCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCt                                                                                                        <  1:281374/127‑1 (MQ=255)                                aTCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCt                                                                                                        >  2:281374/1‑127 (MQ=255)                                   ggCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACg                                                                                         >  2:346838/1‑139 (MQ=255)                                                    gATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          >  1:359226/1‑105 (MQ=255)                                                    gATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACg                                                                                                          <  2:359226/105‑1 (MQ=255)                                                    gATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTc                                                                        >  1:431680/1‑139 (MQ=255)                                                      ttGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTc                                                                        >  2:70911/1‑137 (MQ=255)                                                      ttGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGa                                                                      <  2:251332/139‑1 (MQ=255)                                                             gCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATc                                                               <  1:428110/139‑1 (MQ=255)                                                                 tggctggcCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAAc                                                            >  2:307892/1‑138 (MQ=255)                                                                           cgAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCg                                                       <  2:459626/133‑1 (MQ=255)                                                                           cgAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCg                                                       >  1:459626/1‑133 (MQ=255)                                                                                  cGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACctgctgct                                           <  2:220315/138‑1 (MQ=255)                                                                                             ttgcgtttgcgtTGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTgg                               >  1:482294/1‑139 (MQ=255)                                                                                                  tttgcgttGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCa                                                                              >  2:153293/1‑87 (MQ=255)                                                                                                  tttgcgttGAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCa                                                                              <  1:153293/87‑1 (MQ=255)                                                                                                          gAGCGTTGTAGGCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACt                   >  1:474718/1‑138 (MQ=255)                                                                                                                     gCCTGCTGTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGc       >  1:463807/1‑139 (MQ=255)                                                                                                                          ctgTTCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCt  >  1:576148/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                        TGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCACTATCGTTAACTTCGCCTTCTGCCAACGCTGGGTTAAACAGTACGGTTCAAAACCAGACTTCGAGCCTATCAACTACCGTAACCTGCTGCTGACCATTATTGGTGTTGTGGCACTGATCGCTATCGCCACCT  >  NZ_CP009273/1707475‑1707734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑