Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,708,538	A→G	intergenic (+10/‑96)	dtpA → / → gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,708,538	0	A	G	100.0%	71.8 / NA	23	intergenic (+10/‑96)	dtpA/gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA  >  NZ_CP009273/1708402‑1708649                                                                                                                                         |                                                                                                                cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa                                                                                                               <  2:162125/139‑1 (MQ=255)   tATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAAct                                                                                                             >  1:439079/1‑139 (MQ=255)                tGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCg                                                                                                >  2:556405/1‑139 (MQ=255)                  gtgtCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGc                                                                                               >  2:579481/1‑138 (MQ=255)                  gtgtCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGc                                                                                               >  1:74864/1‑138 (MQ=255)                        cTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAAct                                                                                                             <  1:121068/118‑1 (MQ=255)                        cTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAAct                                                                                                             >  2:121068/1‑118 (MQ=255)                            tGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGt                                                                                    >  2:87870/1‑139 (MQ=255)                                   aTTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGc                                                                             >  2:369367/1‑139 (MQ=255)                                        aGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttt                                                                        <  1:556405/139‑1 (MQ=255)                                                         ccGCGCCGAACCTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCa                                                       <  1:289611/139‑1 (MQ=255)                                                         ccGCGCCGAAACGGCACCGCATGACGCAGGATGACGCTGCAGACAAGGCGGCTAAAGCAGCGGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCa                                                       <  2:98480/139‑1 (MQ=255)                                                               cGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCa                                                 <  1:327634/139‑1 (MQ=255)                                                                           gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                     <  2:249488/139‑1 (MQ=255)                                                                           gCATGACGCAGGATGACGCGGAAGAAAAGGCGGCAAAAGCGGCGGTAGCGAAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                     <  2:439079/139‑1 (MQ=255)                                                                           gCATGACGCAGAATGACGCTGAAGACAAGGGGGGGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                     <  1:162678/139‑1 (MQ=255)                                                                             aTGACGCAGGATTACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                   <  1:272058/139‑1 (MQ=255)                                                                                           cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACg                                                         >  2:302759/1‑103 (MQ=255)                                                                                           cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACg                                                         <  1:302759/103‑1 (MQ=255)                                                                                                cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt                                                                 <  1:516271/90‑1 (MQ=255)                                                                                                cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt                                                                 >  2:516271/1‑90 (MQ=255)                                                                                                cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                >  2:479616/1‑139 (MQ=255)                                                                                                    cAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGaaa            <  1:479616/139‑1 (MQ=255)                                                                                                              aaaGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa  <  1:98497/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACA  >  NZ_CP009273/1708402‑1708649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑