| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,716,352 | A→T | T278S (ACC→TCC) | ydhJ → | HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,716,352 | 0 | A | T | 91.7% | 35.1 / ‑2.8 | 12 | T278S (ACC→TCC) | ydhJ | HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (3/8); total (3/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
TTCGACTCGCCCAACGCGTTCCCGTCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCACCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATT > NZ_CP009273/1716275‑1716464 | ttCGACTCGCCCAACGCGTTCCCGTCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCGCCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCGATTTGccc < 2:449045/139‑1 (MQ=255)ttCGACTCGCCCAACGCGTTCCCGTCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGccc < 2:26800/139‑1 (MQ=255) gCCCAACGCGTTCCCGTCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTc < 1:202908/137‑1 (MQ=255) tCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCa > 1:293834/1‑63 (MQ=255) tCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCa < 2:293834/63‑1 (MQ=255) gATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGccc > 1:21041/1‑101 (MQ=255) gATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGccc < 2:21041/101‑1 (MQ=255) gTCCCAGGCGATGTCACGTTGGTGTCGGGCACCACCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCaa < 1:184740/139‑1 (MQ=255) cAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATg < 1:521504/117‑1 (MQ=255) cAGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATg > 2:521504/1‑117 (MQ=255) aGGCGATGTCACGTTGGTGTCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCa < 2:556803/139‑1 (MQ=255) gCGATGTCACGTTGGTGGCGGGCACCTCCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCAtt < 2:347614/139‑1 (MQ=255) | TTCGACTCGCCCAACGCGTTCCCGTCCGCTTTGCGCTGGATAAAGTCCCAGGCGATGTCACGTTGGTGTCGGGCACCACCTGTAGCATTGCCGTAGGTCAATAATGAACGCATCGTCATGGTCCTTGCGCAATTTGCCCTGGTTCAGGGCCACGCTGGCGCAATGGCGTTATGCGTTACGCAATACCATT > NZ_CP009273/1716275‑1716464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |