Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	243,919	A→T	R241W (AGG→TGG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	243,919	0	A	T	100.0%	134.8 / NA	34	R241W (AGG→TGG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/16);  total (18/16)

AGCCGCGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCG  >  NZ_CP009273/243784‑244052                                                                                                                                        |                                                                                                                                      aGCCGCGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGa                                                                                                                                    >  2:364107/1‑139 (MQ=255)      cgAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGAtttt                                                                                                                                >  1:162191/1‑138 (MQ=255)       gagaTAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGa                                                                                                                              <  2:583129/139‑1 (MQ=255)             gCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACgg                                                                                                                           >  1:373421/1‑136 (MQ=255)                        gggCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTg                                                                                                             >  1:245259/1‑139 (MQ=255)                             tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  1:169102/1‑139 (MQ=255)                             tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        <  1:364107/139‑1 (MQ=255)                             tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  1:50353/1‑139 (MQ=255)                             tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTCGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  2:333837/1‑139 (MQ=255)                             tgtATTTGGGCGATGGGCAATTTATCGAGCCCCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGACCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        <  2:498597/139‑1 (MQ=255)                                         aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGCTAAGGCGt                                                                                            >  1:221882/1‑139 (MQ=255)                                            ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTa                                                                                         <  1:440094/139‑1 (MQ=255)                                                        aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             <  2:232172/139‑1 (MQ=255)                                                        aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             <  1:132163/139‑1 (MQ=255)                                                        aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             <  2:245259/139‑1 (MQ=255)                                                            gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          <  2:162191/138‑1 (MQ=255)                                                                   aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                   >  2:200477/1‑138 (MQ=255)                                                                    ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                 <  2:169102/139‑1 (MQ=255)                                                                     cGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                >  2:271635/1‑139 (MQ=255)                                                                        cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGAt                                                                                                   <  1:46852/101‑1 (MQ=255)                                                                        cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGAt                                                                                                   >  2:46852/1‑101 (MQ=255)                                                                            aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          >  2:148968/1‑122 (MQ=255)                                                                            aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          <  1:148968/122‑1 (MQ=255)                                                                            aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                          >  2:554721/1‑138 (MQ=255)                                                                                         gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                            <  1:55071/139‑1 (MQ=255)                                                                                             aTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                        >  1:276235/1‑139 (MQ=255)                                                                                                   cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGGGCGTCTTATc                                  <  2:50353/139‑1 (MQ=255)                                                                                                     aaCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                <  2:308133/139‑1 (MQ=255)                                                                                                          ttCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                  <  2:162650/132‑1 (MQ=255)                                                                                                          ttCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                  >  1:162650/1‑132 (MQ=255)                                                                                                                    aCCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  2:273391/1‑52 (MQ=255)                                                                                                                    aCCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        <  1:273391/52‑1 (MQ=255)                                                                                                                                   cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg  >  1:4426/1‑139 (MQ=255)                                                                                                                                   cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg  >  1:223355/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      AGCCGCGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCG  >  NZ_CP009273/243784‑244052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑