Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,727,442	T→G	F1367V (TTT→GTT)	lhr →	ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,727,442	0	T	G	100.0%	49.0 / NA	15	F1367V (TTT→GTT)	lhr	ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/8);  total (7/8)

GCTGGACGCTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGTCGTTTTGTAGAAGGTCTGGGTGGCGCGCAATTCGCTGAACGTCTGACTATT  >  NZ_CP009273/1727307‑1727552                                                                                                                                        |                                                                                                               gctggACGCTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGACAATATCCCTGGCGGGGTTc                                                                                                             >  2:161551/1‑139 (MQ=255) gctggACGCTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTc                                                                                                             >  1:146183/1‑139 (MQ=255)     gACGCTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAAT‑TCCCTGGCGGGGTTCCATc                                                                                                         <  1:235620/138‑1 (MQ=255)            gTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGc                                                                                                    <  1:218859/137‑1 (MQ=255)            gTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCa                                                                                                   >  2:108936/1‑138 (MQ=255)                     gCAGGTCGATCCACTAAACGATACCGAAAGGAGGCTTGCTCTGGCGGAAAATATGCACGCCCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGTGGTTCCATCGATGCAAACGCTTTg                                                                                          <  2:445988/138‑1 (MQ=255)                      cAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTc                                                                                        <  2:533893/139‑1 (MQ=255)                       aGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCg                                                                                       <  1:108936/139‑1 (MQ=255)                       aGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCg                                                                                       <  2:319337/139‑1 (MQ=255)                                cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATc                                                                                                         <  1:456896/112‑1 (MQ=255)                                cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATc                                                                                                         >  2:456896/1‑112 (MQ=255)                                         aTACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCgg                                                                     <  1:253739/139‑1 (MQ=255)                                                                 cgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGTCGTTTTg                                              >  2:553905/1‑138 (MQ=255)                                                                                               tcaGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGTCGTTTTGTAGAAGGTCTGGGTGGCGCGCAATTCGCTg                >  1:237581/1‑138 (MQ=255)                                                                                                             gTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGTCGTTTTGTAGAAGGTCTGGGTGGCGCGCAATTCGCTGAACGTCTGACTAtt  >  1:471398/1‑138 (MQ=255)                                                                                                                                        |                                                                                                               GCTGGACGCTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGTCGTTTTGTAGAAGGTCTGGGTGGCGCGCAATTCGCTGAACGTCTGACTATT  >  NZ_CP009273/1727307‑1727552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑