Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,737,390	T→C	E37G (GAA→GGA)	ribE ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,737,390	0	T	C	100.0%	106.1 / NA	31	E37G (GAA→GGA)	ribE	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

TCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAG  >  NZ_CP009273/1737261‑1737525                                                                                                                                  |                                                                                                                                        tCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGt                                                                                                                                >  1:346539/1‑139 (MQ=255)   ccccACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTc                                                                                                                               <  2:346539/138‑1 (MQ=255)      cACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAg                                                                                                                            >  1:161118/1‑138 (MQ=255)                   ccAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                                                                               >  1:350730/1‑138 (MQ=255)                   ccAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                                                                               <  2:350730/138‑1 (MQ=255)                     aaGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAAc                                                                                                            <  2:457057/139‑1 (MQ=255)                                       cGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACg                                                                                           >  2:578562/1‑138 (MQ=255)                                            ctttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAAtt                                                                                     >  1:190466/1‑139 (MQ=255)                                                    aGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTtct                                                                             >  1:358608/1‑139 (MQ=255)                                                      gTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCg                                                                           >  1:111787/1‑139 (MQ=255)                                                      gTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCg                                                                           >  1:497924/1‑139 (MQ=255)                                                       tCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGt                                                                          <  1:579817/139‑1 (MQ=255)                                                                aCATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGaca                                                                 >  1:112991/1‑139 (MQ=255)                                                                aCATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGaca                                                                 >  2:473838/1‑139 (MQ=255)                                                                aCATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGaca                                                                 >  2:417082/1‑139 (MQ=255)                                                                aCATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGaca                                                                 >  2:367887/1‑139 (MQ=255)                                                                           gTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg                                                      <  2:161118/139‑1 (MQ=255)                                                                           gTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg                                                      <  2:98488/139‑1 (MQ=255)                                                                                tttCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGcc                                                 <  1:578562/139‑1 (MQ=255)                                                                                      tCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                                                                               <  1:526866/71‑1 (MQ=255)                                                                                      tCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                                                                               >  2:526866/1‑71 (MQ=255)                                                                                        aCGTTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGACGGGTAACTCCACCTCATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACaa                                         <  2:275323/139‑1 (MQ=255)                                                                                        aCGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACaa                                         <  2:112991/139‑1 (MQ=255)                                                                                                   gcaACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAAc                              <  1:306285/139‑1 (MQ=255)                                                                                                    caACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAAt                                        >  1:480895/1‑128 (MQ=255)                                                                                                    caACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAAt                                        <  2:480895/128‑1 (MQ=255)                                                                                                                    cGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTg              >  2:52317/1‑138 (MQ=255)                                                                                                                      gAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGaaa           <  2:358608/139‑1 (MQ=255)                                                                                                                      gAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGaaa           <  1:367887/139‑1 (MQ=255)                                                                                                                      gAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGaaa           <  1:174713/139‑1 (MQ=255)                                                                                                                               gTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAg  <  1:417082/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAATCCCCGTAAACATACTTACAACTCCTGAAATCAGTTAAG  >  NZ_CP009273/1737261‑1737525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑