Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,037	T→G	V129G (GTT→GGT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,037	0	T	G	100.0%	82.7 / NA	26	V129G (GTT→GGT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/11);  total (15/11)

GTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775907‑1776172                                                                                                                                   |                                                                                                                                        gTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCg                                                                                                                                 <  2:62988/139‑1 (MQ=255)               tCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCgg                                                                                                                   >  2:316627/1‑138 (MQ=255)                                      cGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGt                                                                                            >  1:34527/1‑139 (MQ=255)                                       gCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTg                                                                                           <  2:468849/139‑1 (MQ=255)                                       gCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTg                                                                                           <  1:411171/139‑1 (MQ=255)                                                           tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  2:540537/1‑139 (MQ=255)                                                            aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTg                                                                                           <  1:143933/118‑1 (MQ=255)                                                            aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTg                                                                                           >  2:143933/1‑118 (MQ=255)                                                                  tAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGc                                                                 >  1:70141/1‑138 (MQ=255)                                                                            gACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATgtggt                                                        <  2:222433/137‑1 (MQ=255)                                                                                  tGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTgg                                                >  1:523008/1‑139 (MQ=255)                                                                                       gATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGa                                            <  1:540537/138‑1 (MQ=255)                                                                                              cAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGa                                            <  2:271553/131‑1 (MQ=255)                                                                                              cAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGa                                            >  1:271553/1‑131 (MQ=255)                                                                                                      gCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATg                            >  2:537778/1‑139 (MQ=255)                                                                                                            gggcgCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGc                      <  2:523008/139‑1 (MQ=255)                                                                                                            gggcgCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGGTCGTGAAGGAAACAAGGTCACTGGCGCGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGc                      >  2:212579/1‑139 (MQ=255)                                                                                                             ggcgCGCAGTTTATCCCCGGCGGTCGCGTCGACGCGCTGGTTCGTGACGGAAACAAGGTCACTGGCCTGCAGGCTGGGGATGATATTCTCGAAGCGAATGGTGTGATTCTGGCTGATGGCGTTAACTCGATACCTGGcc                     >  2:581232/1‑139 (MQ=255)                                                                                                              gcgcacAGTTGAGCCCGGGGGGTCTCGTCGACGCGCTGGTTCGTGAAGAAAACAATGTCACTGGCGTGCAGGCTAGGGATGATAGACTCGAAGCGAATGTGGTGATTCTGACTGATGGCGTTAACTCGATGCTTGGCcg                    <  2:203425/139‑1 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCt                                                                                     <  1:503347/56‑1 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCt                                                                                     >  2:503347/1‑56 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  >  2:345474/1‑139 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  <  2:431262/139‑1 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  >  2:576505/1‑139 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  >  1:176509/1‑139 (MQ=255)                                                                                                                                gCGGTCGCGTCGACGCGCTGGGTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  >  1:261314/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                        GTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775907‑1776172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑