Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,541	T→G	V297G (GTG→GGG)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,541	0	T	G	100.0%	35.7 / NA	12	V297G (GTG→GGG)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/5);  total (7/5)

CCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTC  >  NZ_CP009273/1776434‑1776671                                                                                                            |                                                                                                                                   ccccGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGc                                                                                                     <  1:520088/139‑1 (MQ=255)    cGCCATTCGCCCGGTGATTAGCGGCGGCAAACGGATTGAATAATCAGCGCATATGGGGCCGGAGGGCGGTCTGGCAATGGAGCCGCAACTGGTGAACGAGGGCGGGATGGTCGTTGGGGACGCCGCAGGCTTCTGCCt                                                                                                   >  2:520088/1‑138 (MQ=255)                 cTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGtttac                                                                                     >  1:211351/1‑137 (MQ=255)                              gcAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCAt                                                                        >  1:40553/1‑139 (MQ=255)                                              ttCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCa                                                        <  1:93136/139‑1 (MQ=255)                                              ttCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCa                                                        <  2:240465/139‑1 (MQ=255)                                              ttCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCa                                                        <  2:86655/139‑1 (MQ=255)                                              ttCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCa                                                        >  2:93136/1‑139 (MQ=255)                                                ccGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCTGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATc                                                      >  1:521994/1‑139 (MQ=255)                                                ccGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCAt                                                       <  2:165784/138‑1 (MQ=255)                                                                                        gCAACTGGTTAACGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGaa              >  1:224552/1‑139 (MQ=255)                                                                                                    cGAGGGCGGGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTtc  >  1:496824/1‑139 (MQ=255)                                                                                                            |                                                                                                                                   CCCCGCCATTCGCCCGCTGATTAGCGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTCTGGCAATGGTGCCGCAACTGGTTAACGAGGGCGTGATGATCGTTGGTGACGCCGCAGGCTTCTGCCTGAATTTGGGTTTTACGGTCCGCGGCATGGATTTAGCCATTGCATCGGCTCAGGCTGCCGCCACAACGGTGATCGCCGCCAAAGAACGCGCGGATTTC  >  NZ_CP009273/1776434‑1776671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑