Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,801,453	T→G	V276G (GTT→GGT)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,801,453	0	T	G	100.0%	123.1 / NA	34	V276G (GTT→GGT)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/16);  total (18/16)

GTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCA  >  NZ_CP009273/1801330‑1801587                                                                                                                            |                                                                                                                                       gTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGc                                                                                                                         >  2:308996/1‑139 (MQ=255)   gAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCg                                                                                                                             <  2:527467/133‑1 (MQ=255)   gAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCg                                                                                                                             >  1:527467/1‑133 (MQ=255)            aTTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTg                                                                                                              >  2:483678/1‑139 (MQ=255)             ttCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTg                                                                                                              <  1:57130/138‑1 (MQ=255)                     gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAg                                                                                                                          <  2:70249/118‑1 (MQ=255)                     gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAg                                                                                                                          >  1:70249/1‑118 (MQ=255)                     gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca                                                                                                      <  2:224844/138‑1 (MQ=255)                     gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCacac                                                                                                     >  1:540794/1‑139 (MQ=255)                       gCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACt                                                                                                    >  2:530954/1‑138 (MQ=255)                            caccGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt                                                                                                >  2:15557/1‑137 (MQ=255)                            caccGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt                                                                                                >  1:4241/1‑137 (MQ=255)                                ggTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg                                                                                           >  2:575392/1‑138 (MQ=255)                                ggTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg                                                                                           >  1:582853/1‑138 (MQ=255)                                 gTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGaa                                                                                         <  1:252642/139‑1 (MQ=255)                                 gTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGaa                                                                                         <  1:362653/139‑1 (MQ=255)                                    aaaaGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAacac                                                                                      <  2:369033/139‑1 (MQ=255)                                      aaGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGt                                                                                    <  2:540794/139‑1 (MQ=255)                                        gCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCt                                                                                  <  1:271748/139‑1 (MQ=255)                                           agagTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtgtg                                                                               <  1:308996/139‑1 (MQ=255)                                            gagTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt                                                                                                <  1:263915/121‑1 (MQ=255)                                            gagTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGc                                                                              >  1:373339/1‑139 (MQ=255)                                            gagTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCAACACTCAAt                                                                                                >  2:263915/1‑121 (MQ=255)                                                                            gcgcGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca                                                                                                      >  2:36302/1‑83 (MQ=255)                                                                            gcgcGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca                                                                                                      <  1:36302/83‑1 (MQ=255)                                                                              gcgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAcc                                            >  2:470400/1‑139 (MQ=255)                                                                                  tGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtgtg                                                                               <  2:259891/100‑1 (MQ=255)                                                                                  tGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtgtg                                                                               >  1:259891/1‑100 (MQ=255)                                                                                                  aGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                        <  1:127279/139‑1 (MQ=255)                                                                                                        tGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc                  <  2:488196/139‑1 (MQ=255)                                                                                                                ttGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgg          >  2:136837/1‑139 (MQ=255)                                                                                                                        aTGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca                                                                                                      <  2:403294/39‑1 (MQ=255)                                                                                                                        aTGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca                                                                                                      >  1:403294/1‑39 (MQ=255)                                                                                                                        aTGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatca  >  1:307248/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                       GTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCA  >  NZ_CP009273/1801330‑1801587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑