Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,395	T→G	G424G (GGT→GGG)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,395	0	T	G	100.0%	88.3 / NA	25	G424G (GGT→GGG)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/9);  total (16/9)

GCTGAAAACGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGA  >  NZ_CP009273/1809273‑1809523                                                                                                                           |                                                                                                                                 gCTGAAAACGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCAt                                                                                                                   <  2:577413/138‑1 (MQ=255)             cAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTa                                                                                                      <  2:539563/139‑1 (MQ=255)                        ccATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGa                                                                                                                           <  1:200741/107‑1 (MQ=255)                        ccATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGa                                                                                                                           >  2:200741/1‑107 (MQ=255)                         cATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGAcc                                                                                           <  1:527225/138‑1 (MQ=255)                                     atCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCAt                                                                               >  1:387555/1‑138 (MQ=255)                                     atCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCAt                                                                               >  2:195654/1‑138 (MQ=255)                                                 ctggactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTg                                                                  <  2:520242/139‑1 (MQ=255)                                                    gactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                                                               >  1:346277/1‑139 (MQ=255)                                                         cACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGgcatg                                                          >  1:480718/1‑139 (MQ=255)                                                                gATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCa                                                   <  1:265574/139‑1 (MQ=255)                                                                  tCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATg                                                 <  2:387555/139‑1 (MQ=255)                                                                   ccGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATgg                                                >  2:303694/1‑139 (MQ=255)                                                                         ttGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGAt                                            <  1:303694/137‑1 (MQ=255)                                                                                 aCGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAc                                  >  2:7729/1‑139 (MQ=255)                                                                                  cGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAcc                                 <  2:469648/139‑1 (MQ=255)                                                                                   gTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAAccc                                >  2:339524/1‑139 (MQ=255)                                                                                                     aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTACCCCGGCGAATTAcgaaccgaa              >  1:257244/1‑139 (MQ=255)                                                                                                     aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa              >  1:235170/1‑139 (MQ=255)                                                                                                     aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa              >  2:103590/1‑139 (MQ=255)                                                                                                     aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa              >  1:281938/1‑139 (MQ=255)                                                                                                     aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa              >  1:245239/1‑139 (MQ=255)                                                                                                             aTCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGAtt       >  1:53788/1‑138 (MQ=255)                                                                                                                aGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAAc    >  2:187724/1‑138 (MQ=255)                                                                                                                  tcgtcTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGa  >  1:344913/1‑138 (MQ=255)                                                                                                                           |                                                                                                                                 GCTGAAAACGAACAGGCGGCTTTCCATCCTGGGCATATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGA  >  NZ_CP009273/1809273‑1809523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑