Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,834,793	A→G	D357G (GAT→GGT)	ynjE →	thiosulfate sulfurtransferase YnjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,834,793	0	A	G	100.0%	43.2 / NA	14	D357G (GAT→GGT)	ynjE	thiosulfate sulfurtransferase YnjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)

CGTTCGTGGCCAGAATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCA  >  NZ_CP009273/1834663‑1834924                                                                                                                                   |                                                                                                                                    cgttcgtGGCCAGAATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCAcc                                                                                                                              <  1:10786/138‑1 (MQ=255)  gttcgtGGCCAGAATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCAt                                                                                                                            >  2:287825/1‑139 (MQ=255)                                             tatTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGaa                                                                                  >  2:340912/1‑138 (MQ=255)                                                                                                  aCTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTc                            >  1:367933/1‑139 (MQ=255)                                                                                                    tCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTcc                           >  2:86687/1‑138 (MQ=255)                                                                                                      gACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGa                         >  2:377917/1‑138 (MQ=255)                                                                                                       aCGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGaaa                       >  1:54623/1‑139 (MQ=255)                                                                                                              tGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTAt                >  2:194839/1‑139 (MQ=255)                                                                                                                  aGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTac            >  2:359269/1‑139 (MQ=255)                                                                                                                     ttttCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTacgca         <  2:470860/139‑1 (MQ=255)                                                                                                                     ttgtCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTacgca         <  2:154530/136‑1 (MQ=255)                                                                                                                        tCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTacgcacgc      >  1:246999/1‑139 (MQ=255)                                                                                                                             aCCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCa  <  1:287825/138‑1 (MQ=255)                                                                                                                             aCCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCa  <  1:86687/138‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CGTTCGTGGCCAGAATTTATCGGTACGACCAGCGGTTACAGCTATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGCGGCACCGGCTGGCGCGCGTCCGAAACCTTTATGTACGCACGCGCCA  >  NZ_CP009273/1834663‑1834924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑