Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,856,434	A→G	S85P (TCA→CCA)	msrB ←	peptide‑methionine (R)‑S‑oxide reductase MsrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,856,434	0	A	G	100.0%	60.4 / NA	18	S85P (TCA→CCA)	msrB	peptide‑methionine (R)‑S‑oxide reductase MsrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/11);  total (7/11)

AAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACG  >  NZ_CP009273/1856317‑1856561                                                                                                                      |                                                                                                                                aaGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAAt                                                                                                            <  2:380446/139‑1 (MQ=255) aaGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAAt                                                                                                            <  1:158054/139‑1 (MQ=255)      gCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGAtt                                                                                                       <  2:13335/139‑1 (MQ=255)        agagTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGAttct                                                                                                     <  1:488107/139‑1 (MQ=255)                     tAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGtt                                                                                        >  1:506199/1‑139 (MQ=255)                     tAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGtt                                                                                        >  1:574610/1‑139 (MQ=255)                         gTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTa                                                                                    <  2:437194/139‑1 (MQ=255)                              cccGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAAc                                                                               <  2:574610/139‑1 (MQ=255)                                    ggCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGgc                                                                          <  2:159534/138‑1 (MQ=255)                                       tGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGgccagc                                                                      >  2:188353/1‑139 (MQ=255)                                                         aCATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACt                                                    <  2:195329/139‑1 (MQ=255)                                                                                 ttACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCgggg                            >  1:501030/1‑139 (MQ=255)                                                                                      gCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCg                       >  1:464440/1‑139 (MQ=255)                                                                                                tCTATGCGCTGCATTCCATGTGGCAAGTCGTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCaaa              <  2:501030/138‑1 (MQ=255)                                                                                                    tGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACa            <  2:116157/136‑1 (MQ=255)                                                                                                    tGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACa            <  2:111574/136‑1 (MQ=255)                                                                                                         tGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGAtata    >  2:274682/1‑139 (MQ=255)                                                                                                           cATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACg  >  2:19165/1‑139 (MQ=255)                                                                                                                      |                                                                                                                                AAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACG  >  NZ_CP009273/1856317‑1856561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑