Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,900,350	A→G	T136A (ACC→GCC)	mntP →	manganese efflux pump MntP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,900,350	0	A	G	100.0%	59.3 / NA	18	T136A (ACC→GCC)	mntP	manganese efflux pump MntP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

GCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAG  >  NZ_CP009273/1900218‑1900484                                                                                                                                     |                                                                                                                                       gcAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCAt                                                                                                                                  <  1:123450/139‑1 (MQ=255)          gaagatgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTg                                                                                                                         >  1:167539/1‑139 (MQ=255)               tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTtgt                                                                                                                    >  1:357055/1‑139 (MQ=255)               tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTtgt                                                                                                                    >  2:212599/1‑139 (MQ=255)                        gcgcCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTg                                                                                                           <  2:214204/139‑1 (MQ=255)                                      gTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACAtt                                                                                             <  1:550009/139‑1 (MQ=255)                                             gCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGga                                                                                        <  2:357055/137‑1 (MQ=255)                                                    gTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGcc                                                                                                                             <  1:247565/93‑1 (MQ=255)                                                    gTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGcc                                                                                                                             >  2:247565/1‑93 (MQ=255)                                                    gTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGAtggt                                                                                 <  1:515173/137‑1 (MQ=255)                                                              cgATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTAt                                                                     <  1:295441/139‑1 (MQ=255)                                                                   gCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCt                                                                >  2:38973/1‑139 (MQ=255)                                                                                         cTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCgg                                           >  2:464040/1‑138 (MQ=255)                                                                                                  ttGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTcgg                                 <  1:212599/139‑1 (MQ=255)                                                                                                                      gTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCgg                                                                  >  2:568893/1‑86 (MQ=255)                                                                                                                      gTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCgg                                                                  <  1:568893/86‑1 (MQ=255)                                                                                                                                tCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGG‑AAAAAGCGGAAATTCTTGGCGGGCTGGGGCTGATCGGCATCGGGGTc     >  2:411247/1‑136 (MQ=255)                                                                                                                                  gcgGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAg  <  1:464040/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAG  >  NZ_CP009273/1900218‑1900484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑