Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,955,016	A→G	D233G (GAC→GGC)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,955,016	0	A	G	100.0%	89.1 / NA	26	D233G (GAC→GGC)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

AAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCA  >  NZ_CP009273/1954887‑1955135                                                                                                                                  |                                                                                                                        aaGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                                                                <  2:402157/139‑1 (MQ=255) aaGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                                                                >  1:410358/1‑139 (MQ=255) aaGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                                                                <  2:249093/139‑1 (MQ=255)     tttCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                            >  1:566424/1‑139 (MQ=255)     tttCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                            <  1:70587/139‑1 (MQ=255)           ccGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAg                                                                                                         <  2:410358/136‑1 (MQ=255)                aTGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAAc                                                                                                      >  2:141042/1‑134 (MQ=255)                aTGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGAt                                                                                                 <  1:396553/139‑1 (MQ=255)                         aGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                                                        <  2:345561/139‑1 (MQ=255)                                              agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                   <  1:40927/139‑1 (MQ=255)                                              agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                   <  1:99334/139‑1 (MQ=255)                                               gTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAcc                                                                  >  1:209980/1‑139 (MQ=255)                                                ttCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGtct                                                                                  <  1:417490/122‑1 (MQ=255)                                                ttCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGtct                                                                                  >  2:417490/1‑122 (MQ=255)                                                  cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                                               >  1:5328/1‑139 (MQ=255)                                                  cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                                               >  1:50943/1‑139 (MQ=255)                                                  cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                                               >  2:44807/1‑139 (MQ=255)                                                  cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGCGACGCTGACCCGt                                                               >  2:418715/1‑139 (MQ=255)                                                             cACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGAt                                                    <  1:505708/139‑1 (MQ=255)                                                                       cGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGc                                          <  1:276278/139‑1 (MQ=255)                                                                          ggTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                   <  1:18459/111‑1 (MQ=255)                                                                          ggTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                   >  2:18459/1‑111 (MQ=255)                                                                                    cAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAAcc                                >  2:102113/1‑136 (MQ=255)                                                                                    cAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAAcc                                <  1:102113/136‑1 (MQ=255)                                                                                                gAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGaa                 <  2:444737/139‑1 (MQ=255)                                                                                                               aTGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCa  <  1:71910/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                        AAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCA  >  NZ_CP009273/1954887‑1955135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑