Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,000,880	A→G	D415G (GAC→GGC)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,000,880	0	A	G	100.0%	29.3 / NA	10	D415G (GAC→GGC)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

TACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT  >  NZ_CP009273/2000747‑2001008                                                                                                                                      |                                                                                                                                 tACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATc                                                                                                                             >  2:95750/1‑139 (MQ=255)                       tCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCg                                                                                                       >  1:24135/1‑139 (MQ=255)                       tCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCg                                                                                                       >  1:572221/1‑139 (MQ=255)                                aCGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCa                                                                                               <  1:306145/138‑1 (MQ=255)                                                 atagataTGCCAATAATCGAACAGCTTGGTGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCt                                                                             <  1:95750/139‑1 (MQ=255)                                                 atagataTGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCt                                                                             <  1:511421/139‑1 (MQ=255)                                                                                                                     aCAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCaa          >  1:535427/1‑138 (MQ=255)                                                                                                                          cGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCgg                                                                               <  1:26635/64‑1 (MQ=255)                                                                                                                          cGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCgg                                                                               >  2:26635/1‑64 (MQ=255)                                                                                                                            ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt  >  1:111226/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                 TACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT  >  NZ_CP009273/2000747‑2001008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑